Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314020611_20x67i5e CATCATCAACAGGAAGGTCCTAGGCAGCCGGGTGTTTGGGAATAA(-GGTAAACAATA)ACTTCCTAAGGGCTCCTAAGCCGCTACTAGACCCAGGGGTCAGGG NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x671ax TCACTTTAGTGATATTCGGTGTGCTACAGTCAGGTGGTCCTCAAA(-GGCCTTTCGGG)ACAAAGCCCTTGGGATGATTAACAGACCTACAGAGAACAAAAAGC NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x66uhm TTACCTAATGGACAGGGAGAACATTTGTTTACTGTTCGCATAGTA(-GGCAGGTCAAG)ATCAATCTGTGATAGAATATCGGAGTTGTATTTGACTTTTTGAAA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x652zg TTACGTGATTTGACCATCACACATATCGTTGACTCAAACTTTGGT(-TTATCTTTCTA)GAATTTGTTACTCTTTTTTCCTAGTGAAAATATATCCTTTTTATT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x64lkn TTTTGGGAATCTTTTTAAGTAGTGAACCTTTTGTACCATGTTAGG(-AAGTCTGTAAA)AGTTGAGATTTTGAAATGTTTCGGTCATCAGCAGAAAAGTTAATA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x64a9z TCAAGCCTCCTGTTTTTAAATTAAGGCAATCCTTGGTGATCAAAG(-GATAGTAGGGG)AAGTGAGTAATAAATATAAGTCAAGCAATCATAGAAATGTGAAAA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x63qcy AATATTGTTTGGAGTCAGGATATTAGTTTCTGAAGTTGGTTACAG(-AATGAGGAAAC)AACCCCAGAGAAAGCAGTAAGACTCCCTCAAGATTACAGAAGATT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x63e6b CACCACCCTGCCACCAGGGACCATCCAATACCTGACTGACACCTC(-CAAGTGGGTAC)CATCCTGCCTCCATTGCACACACCCACCTTCCCGCCCCACCCTGT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x62jy3 ATGACACTGTGTAAGATTTTATTCTGAGGTTCTGACACCTGCATA(-TGGTGGATGAA)GGCGCTCAAGCCTTTGAGAATTGAAGGCAGTGCTTAAGTGGATGA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x61l48 TTAGTAGTATTGCTCGAGATATGTAAAGTGTTCATCTCCTTTTGA(-TTGTGGAAGGG)ACAAAGGGACAGAGTCCAAGTGGTTCATATTAGAAATTGACTAGA NGG 10 8 17 8 24 0 100 5 1