Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314015620_u37dyemk GCGGCAGCTCTGAGCCCAGGCGCTTTCCTTAGACTTGCAGCGATG(-CACGGATTGCA)TGGGTGGGGGGCGGGGTGGGGAATGAATGCCGGTGCATGCACTTT NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dxsl9 TTACAACCACGGGAATAAAAACAGAGAAGATGCCTACCTTCATGG(-AATTTACAGTC)TTATTTTGTTTTATAGAAATCATGTGTCCTAGTAATCAAATGGGG NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dxjoc GAAGCCTCTTAATCTTGGAGTGACTTTGGAGATTTTAATGGCAGT(-AGGTAATTCCT)TTACTGTTACTGTTGGCAGAATCTAAAGTTGAAGGTCCCTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dxhtl TCGGGTAAGGGGTTACATCCGGGAAACTCGTCAGGGAAATGGCGG(-GAGATGCTGAG)GGTGCGGGGCCTTTGGAATTCCTTGTTCCTTGCCCTCACCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dvird GAAGCTTGGGGTGTTTCAAAGAGATTCTTTTCGTGTTTTTGTGGT(-ATTTGGGTTTA)AGGGAGTGTACTGTTTGTTGCTGCAAACTACTGGCTTTGTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dvgy4 TCTCCCGTTTTGTTTTAGTATTAGTGGAAGAAACTGGATTGGATA(-TCAGATCTGTT)TGAAAATGTATTTTCACATGGATCATTTTTTATTAATCTTTGAAT NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dv95t GTGTATGAAGATGCCATGACAGTGTTATGAGTGTGTTGAATGGAG(-ATAAAAGTAGC)ACGTTCAAGTGGACCTATATTCATGTTAAAGTAGTTATAAACTAT NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37duqy8 AATATTGTTTGGAGTCAGGATATTAGTTTCTGAAGTTGGTTACAG(-AATGAGGAAAC)AACCCCAGAGAAAGCAGTAAGACTCCCTCAAGATTACAGAAGATT NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dthk3 GAATGGTGGGTTGGATGCTCTGGAAATTAAGAGGGACCATCTCAT(-GGACATGATTC)CCTGCGGGTAACCCCAACACAGAGGTGGAGAAACGCGGTGGTAAC NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dtdca TCGTACCGATTCCTTCAGGGCGAAAAGCGTGGGGCCGTGCGGAGG(-GAGTAGCGGCG)CGGCCCCAGCATGCACCTGGCCTGAGGGTTGTTCCCGGCAAGCAC NGG 10 8 17 8 24 0 100 5 10