Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314015620_u37dt7qq GATAAGGACAGGCCTGGGGAATGGGGATGATATGTGTGGCATGTG(-AGGTAAATTGG)GTAAATGACAGACTATTTTATATTTGTGACTTACGCGTCTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dt2ak CACCACCCTGCCACCAGGGACCATCCAATACCTGACTGACACCTC(-CAAGTGGGTAC)CATCCTGCCTCCATTGCACACACCCACCTTCCCGCCCCACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dq9o0 TCACTTTAGTGATATTCGGTGTGCTACAGTCAGGTGGTCCTCAAA(-GGCCTTTCGGG)ACAAAGCCCTTGGGATGATTAACAGACCTACAGAGAACAAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dpup1 GTGAGTGTCCCTGTACCCACCCCACAGCACTGGCAGGGCCCACAC(-CACCACGGGAC)AGGCCCAGTCCCCGCCATGCAACCTCTGCCTGGGCCAAGGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dp0ri GATATGTTGCCCGAAAGGGCCTTACTTAGGCATGATGCATCTCGG(-TGTTAGGAAGC)CTCTGCAGAGGGGTAGTTTGGTAGAGGTGACGTGTGTATGCATGG NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37do56n GCGGCCGCGCCCCGCAGCTCTGTAGGCCCTGAAGCTCGGGCTAGG(-AGAGTGGTCCG)CAGGTGGTACCGCACGGGAAGGGACGGATGCGCGGCCGAGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dkzxx ACCTCCTAAAGGAGGCAGCATTGGGGGGTTGGGGGGCGGAGTCTG(-AGGGAGGAGAG)GGTAATCAGGTGGGCGCGTTCCGGGGGCCGAGCGCGGCCTCGCGG NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37dko7k CATCATCAACAGGAAGGTCCTAGGCAGCCGGGTGTTTGGGAATAA(-GGTAAACAATA)ACTTCCTAAGGGCTCCTAAGCCGCTACTAGACCCAGGGGTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37djyf3 AGAAGCTGATGTGCTAAATGAGGGGTGGCAAGAGCTTTAAATAAG(-GGAACTGAGAG)AAGAGTAAGTGAAGGGAACAGAAATTGTTGAGGTATGAGGTCATT NGG 10 8 17 8 24 0 100 5 10
    240314015620_u37djfsc TGAACGGCTCCCAGAACCTTGTTCGTGTGTATGTGTGTAGACAAA(-ATGGCGCCGCG)GCCTTCTTCTGCGCCCGCCATCATGGCTGCCTGCTGTAGGGAGGG NGG 10 8 17 8 24 0 100 5 10