Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314015439_vky7z83c TGAACGGCTCCCAGAACCTTGTTCGTGTGTATGTGTGTAGACAAA(-ATGGCGCCGCG)GCCTTCTTCTGCGCCCGCCATCATGGCTGCCTGCTGTAGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7z0oc GGGCTCTCACGCAACTTTGGGGAGCAACCACAGGCTTTTCTTTTG(-GTGTGTTATAG)AGACCCTCATCCATCTGGCATGGTTAGGAAATGAAAAAATTGGTT NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7yzpk GTGTATGAAGATGCCATGACAGTGTTATGAGTGTGTTGAATGGAG(-ATAAAAGTAGC)ACGTTCAAGTGGACCTATATTCATGTTAAAGTAGTTATAAACTAT NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7yrb3 GCTGGTGAGTGTGCCCAGACCTCCCAGCATCCATGGCCAGCCGGG(-GAGGGTTCGGG)AACACACAGACCCACACACAGGCTCAGGAAAGCATGGGGGTCAGA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7ylt2 GAATGGTGGGTTGGATGCTCTGGAAATTAAGAGGGACCATCTCAT(-GGACATGATTC)CCTGCGGGTAACCCCAACACAGAGGTGGAGAAACGCGGTGGTAAC NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7yft8 TACTTATCAAGTAGTGACAGTAGCTTAAGATACTAGCATCCTGGC(-TGAAGAATGAA)ATCATAAGAATGATGAGTTAGGTTATACTCCCATTAAGTACATCT NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7xzg7 TCGTACCGATTCCTTCAGGGCGAAAAGCGTGGGGCCGTGCGGAGG(-GAGTAGCGGCG)CGGCCCCAGCATGCACCTGGCCTGAGGGTTGTTCCCGGCAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7xss9 CTGGACAGTATAAAGACAACCCAGTATCAGATAACTGGGATGGAC(-CCATTTGAAGG)ATAGTCTGGTTGTGGATTGGTAGCACGTGGTAGTTTCTGTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7xq9z TACATACAGTAGGTTTGTACCTGGGATATATACCTTTTATAGGCA(-TCATGTGTAAT)CTTTGGGCAAAATTGATGCCGTTACATGGTATTTGGTCTGTGGTA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7xjep GGAGCTCCATTTGGTATGTTTGTTTTGTTTTCCGGCCAAAAAATA(-ATGTGCGTGTT)GCCTGGTGATAGCATGACCTTTTATTGCAGTTTCAAGTTGTCTAC NGG 10 8 17 8 24 0 100 5 10