Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314015439_vky7noau ATCATAGGATTAGACTTGGTAGATACAAAGGAATTTTAAGGTAGA(-CAAGGAATTTA)CATTTTTGTTTGAAGAACTGGCTTATATTTTTAAAACAAATTGGA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7n1lt CTATATGTATTTACATCCATCTCTGTCCACATACACCATTCCTAA(-GGACCGGAGGG)TGCGTCTCCTAAAATGACGGCCACCCCTCGCATGCACCCACCGTC NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7muc0 TTGATTGGAGTGTGTTGTACATGTGATTGAATGTTATAGAGGCAG(-AAAAAAGATGA)AGAGCTCTAAATTTCTGCCCTCTGGTAAGCTTTTTAGAAAAATAT NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7ldgs TTGACAAACCCAGACACGCCGGGTCCAAGCACCCTTCTCCCATTC(-CCCAAAGTCGT)GCCCATCCTCCTGCCCTGCCCTGCTGCACCCAGTTTTCTCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7kh4v CCGTCGATCCAGAGTCCCTCCACCGTGGTCCTGGAGTCCCCCTCA(-CCCTTGACCCG)GATCCACCCCCACCGTGGAACCAGGGTCGACGCCACCGTGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7k0l4 CCATGTTTTCGCTGTAACTGCTCTGCTACCTTGGGAACCTGGCAC(-CTTGCATGACT)TTGTCCACCTGGCATCCCACTATATGCACTTGTACTTAGGCTGAT NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7jc7d AAGCTAGAAAAGAATTGTGCATAAATTTGTGTTCTTATTGCTGAA(-TATTTCCTCAG)AATACCTTTTTTGAATATTCGAAGTGACATTTGTTTATTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7j5fb TAGGATGAGAGGCAAGGACAAGCTCAGGGGTTTGGCCTGGATACC(-TGGTTTAGAAT)GAAAGTAGGCTCATTCCCAAGGAAGGAGATGAGCAAGCAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7iy7w TACAAAAGGCTGCATGTTATGTGATTCCACTTCTTTGTCTTTCTG(-GAAAAGGTAAT)ACTATTGAATAAAAATGAGGAGGTAGAGAAGAAGGTACTTCACTT NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7ilxj ATAATTCGTATTCCAGAAGTAGTAAAAAGACCTCAAGTTTAAATT(-TTCTCTGTTTT)AGCCTCAAATACAATTCATTCAGCATGCTGTTATCAGTTATTTTA NGG 10 8 17 8 24 0 100 5 10