Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314015439_vky7c5w1 ATTCGTTCCACACTTGGCCAGGGAACTGGGGGCTGAGGATACTTT(-GGCTTTGGTCT)TTGGGATTTCAAGGGAGGAGTTTAGGTCTATGACCCCCTCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7bmia CCCAAAAGGAGCCTACTGCCTTTCACCTCTTGGTGCAATTCTCAT(-GTGGAAAAAGA)TGCAGAGCTCCTTTTCTTGTTCTCTAGGCAGAGGAAAGTAGAGTA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7b3xb ACCTCCTAAAGGAGGCAGCATTGGGGGGTTGGGGGGCGGAGTCTG(-AGGGAGGAGAG)GGTAATCAGGTGGGCGCGTTCCGGGGGCCGAGCGCGGCCTCGCGG NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7adph TCAGAGTTATAAGGAATTGTTTTCCTTAGAAAACTTAACATAAAG(-GGGAAAGTAAA)AATTCTGTTGTCCGAACACTGCCTTTGACCTTATACTACGAATTT NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky7a7yp TCAAGGACTTCTTGGCAGGTGGGGCCCTAGAGCTCAGATGTGGAG(-GAAGAGGGAGG)AGCTGGGTGTGGGCAGGAGCAGAGGGTCCTAGGCTGAGGGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky79cn0 CCCGAGCCCGGGGGAGGAGGGGAGTGCCCGTGTCCGTGTTGGGGT(-TTGAGGGGTCG)GGAGCGGCGTTGGTGGCGCGAGGTGTGAGGCTGCCCGCCGACGGA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky78w42 CCTATGAAATATCAGGTAATGTTCATGAACAGAATACATTAAGGA(-ATCAGAATCAG)TGTAAAGATTTTTAAGTATCCGGGCGCGGTGGCTCACACCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky78lgh AGAGATCTACCAGACATCCATAGATCCCACTCCTTCCTTTAAAGC(-ATGGGAAAACT)GATATCTAGAGGAATTAAGGGATTCGTCCATGGGATACTGCTGGT NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky78kyq AAATAAAATTTAATATGAGAGGGCAGACCTTGAAAAGGTGGTGGG(-AGGTGGTCCTG)TGGTTTTTTTTTTTTTTTTAAATCACATGAAATATTTCAAAAATA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky78kp6 GAATATAGTAGGCATATGTATAATCTAATGTTAAAATAAGTTATA(-TAATGGGTAAT)AATTTTTAGATTTCTTAGATAAGTTACACATTCCCAGGATGAGGT NGG 10 8 17 8 24 0 100 5 10