Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314015439_vky77zkz GATATGTTGCCCGAAAGGGCCTTACTTAGGCATGATGCATCTCGG(-TGTTAGGAAGC)CTCTGCAGAGGGGTAGTTTGGTAGAGGTGACGTGTGTATGCATGG NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky77uuz CAGGTAGCCTCACAGCGCATCTGTTTGTTGTGAAAGCCGAGCTCT(-TACTCATGTGA)CCTGCTCCTCCCCCGCGGCCGGCGGAGGGGGACGGCCGGCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky77ex8 TTCAGAACAGTGAGGAACTGATATTTAGTCATTTCTGTGCACCCC(-TGGTCACGCAG)TTGCGCCCCCCCCCCCTTTTTTTTCCTTAACTTTGTTGTTTTTTG NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky759j8 AAGAGGCAGATAGAGGAGCATTTGAGTCTGGGTGTTTTTGGAGGC(-TTCTCATACCT)GATTGGTCCCTATAATTATGAGGAAGAGAGGGATGGACATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky74zsp CTGGGGTACAAAAACCCTAGGCCCACAAATCCAGTGAGGTTTCTT(-CCATCATACTG)ACCCTTCTCCTTTGACTCCCTGGGTAGAGTTCAGCCCCTACCCCA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky74kx6 CAGCGGGTTTGGGGGGATTACCGGCCTGCTGCACGCCCACCCCAG(-CCCCTCAGCTG)CTGCCCCATCCCGAGGCCCCCATCCAGGCTCCCCAGCCCGCAGTC NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky748dz CCACTTGCTTACTGAGGAATTTCCCCATGAAGAGCCCTCCCCCTG(-TGAAATGTGGC)CTTGCTGGCATTTGAGAACTGACAAAGGGTGGAGTGAATCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky73vpm TAAATTTATGAAATTAAGCTACAATATGAAACTTGAACATGGATT(-TATTATAGTTA)AAATTCTTTAGGTACATGTAACCAGAAGTTTATATATCAAGTAAA NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky73scg TCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCGGGCCG(-ATCTTATTTTT)TTGTGTCCACAGCATGGATTTCCCTGGTGATCCACCTGCCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240314015439_vky73sbn TCCAGACCGGTGGGACTCTGAGCATGCGGGAGGGGTCAAGAAGGC(-AGGGCTGTGGG)TAGGCTCGAGCAGCCAGGGAAGGCTTCCAAGGCAAGGCGGGCTCA NGG 10 8 17 8 24 0 100 5 10