Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314001511_kr4ys8ax AGGTTGTCTGTTCCAAGGTATGCATTGTATTCATCTGTGTAATGG(-ATTTAGGTGAA)GGTAGTCATGTAGTTGCTTTGAGATTTTATTTTTTTGCTAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4yqasb ATATTCTTGGTTAATATTTGTACTTACTCAAGTTTCTCCCCCCAC(-CTTCCGTCCCA)TCTCTCACTCACATTGTGGAATGTGTTACTTTGTGTTGTTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4ypjid TTCAGAACAGTGAGGAACTGATATTTAGTCATTTCTGTGCACCCC(-TGGTCACGCAG)TTGCGCCCCCCCCCCCTTTTTTTTCCTTAACTTTGTTGTTTTTTG NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4yp715 TCAAGGACTTCTTGGCAGGTGGGGCCCTAGAGCTCAGATGTGGAG(-GAAGAGGGAGG)AGCTGGGTGTGGGCAGGAGCAGAGGGTCCTAGGCTGAGGGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4yo9l1 GATATGTTGCCCGAAAGGGCCTTACTTAGGCATGATGCATCTCGG(-TGTTAGGAAGC)CTCTGCAGAGGGGTAGTTTGGTAGAGGTGACGTGTGTATGCATGG NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4yns1q CACCACCCTGCCACCAGGGACCATCCAATACCTGACTGACACCTC(-CAAGTGGGTAC)CATCCTGCCTCCATTGCACACACCCACCTTCCCGCCCCACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4yn1ak TCAGAGTTATAAGGAATTGTTTTCCTTAGAAAACTTAACATAAAG(-GGGAAAGTAAA)AATTCTGTTGTCCGAACACTGCCTTTGACCTTATACTACGAATTT NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4ymgtx TTAGTAGTATTGCTCGAGATATGTAAAGTGTTCATCTCCTTTTGA(-TTGTGGAAGGG)ACAAAGGGACAGAGTCCAAGTGGTTCATATTAGAAATTGACTAGA NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4ylrl9 ACCTCCTAAAGGAGGCAGCATTGGGGGGTTGGGGGGCGGAGTCTG(-AGGGAGGAGAG)GGTAATCAGGTGGGCGCGTTCCGGGGGCCGAGCGCGGCCTCGCGG NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4yll25 ATAATTCGTATTCCAGAAGTAGTAAAAAGACCTCAAGTTTAAATT(-TTCTCTGTTTT)AGCCTCAAATACAATTCATTCAGCATGCTGTTATCAGTTATTTTA NGG 10 8 17 8 24 0 100 5 10