Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314001511_kr4yb4d7 CTTGGTGATTCTGATTTCCTGACTTGGAGTGGATTAAAGCAGGAA(-ATTAAGAGGGA)GTCAGGCTTTTTTTTTTTTTTTTTAGGCAGTATCTGTCTCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4ya5ae TTACAACCACGGGAATAAAAACAGAGAAGATGCCTACCTTCATGG(-AATTTACAGTC)TTATTTTGTTTTATAGAAATCATGTGTCCTAGTAATCAAATGGGG NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4y9sfs GAAGCCTCTTAATCTTGGAGTGACTTTGGAGATTTTAATGGCAGT(-AGGTAATTCCT)TTACTGTTACTGTTGGCAGAATCTAAAGTTGAAGGTCCCTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4y9bnk GTGAGTGTCCCTGTACCCACCCCACAGCACTGGCAGGGCCCACAC(-CACCACGGGAC)AGGCCCAGTCCCCGCCATGCAACCTCTGCCTGGGCCAAGGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4y8ntc TCGGGTAAGGGGTTACATCCGGGAAACTCGTCAGGGAAATGGCGG(-GAGATGCTGAG)GGTGCGGGGCCTTTGGAATTCCTTGTTCCTTGCCCTCACCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4y5t47 CCCGAGCCCGGGGGAGGAGGGGAGTGCCCGTGTCCGTGTTGGGGT(-TTGAGGGGTCG)GGAGCGGCGTTGGTGGCGCGAGGTGTGAGGCTGCCCGCCGACGGA NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4y5fun TTACGTGATTTGACCATCACACATATCGTTGACTCAAACTTTGGT(-TTATCTTTCTA)GAATTTGTTACTCTTTTTTCCTAGTGAAAATATATCCTTTTTATT NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4y56ph CTGGACAGTATAAAGACAACCCAGTATCAGATAACTGGGATGGAC(-CCATTTGAAGG)ATAGTCTGGTTGTGGATTGGTAGCACGTGGTAGTTTCTGTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4y3mnq CAATATAGTAGTAGCTGCATATGATTGAAAATTTACTAGGTCACT(-AGTGCACCAAA)AATTTTATTCACAGCCTCCAGGCATCTTTTGAAATGTGCAACAAA NGG 10 8 17 8 24 0 100 5 10
    240314001511_kr4y0xz8 TCACTTTAGTGATATTCGGTGTGCTACAGTCAGGTGGTCCTCAAA(-GGCCTTTCGGG)ACAAAGCCCTTGGGATGATTAACAGACCTACAGAGAACAAAAAGC NGG 10 8 17 8 24 0 100 5 10