Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314001308_b6uc4ew5 CTGGACAGTATAAAGACAACCCAGTATCAGATAACTGGGATGGAC(-CCATTTGAAGG)ATAGTCTGGTTGTGGATTGGTAGCACGTGGTAGTTTCTGTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240314001308_b6uc3vmk TTTTGGGAATCTTTTTAAGTAGTGAACCTTTTGTACCATGTTAGG(-AAGTCTGTAAA)AGTTGAGATTTTGAAATGTTTCGGTCATCAGCAGAAAAGTTAATA NGG 10 8 17 8 24 0 100 5 10
    240314001308_b6uc3fyz CAGGTAGCCTCACAGCGCATCTGTTTGTTGTGAAAGCCGAGCTCT(-TACTCATGTGA)CCTGCTCCTCCCCCGCGGCCGGCGGAGGGGGACGGCCGGCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240314001308_b6uc0yr7 ACAGGGCATCCCCTGGGCCTGGCTGGGGCCACCAAACCATATGTG(-GCAGAGCCAGG)ATAGGAACGGAGCCTGCCCTACTAACTCCCTTTCTGGACTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240314001308_b6uc01pm AAATAAAATTTAATATGAGAGGGCAGACCTTGAAAAGGTGGTGGG(-AGGTGGTCCTG)TGGTTTTTTTTTTTTTTTTAAATCACATGAAATATTTCAAAAATA NGG 10 8 17 8 24 0 100 5 10
    240313230209_6tt4 AGCTTCTATGATCCTCTTTGGAGCTTAATTCTTAATCCCTTTATTTTCACTTGCTTGTTGATAACAAAGAAGAACTAATTATTAATTTATTTCAAAATGCATGTATTATATTTGATGGGCCACACTAACAGTTATAAACCAAACAACAGATTGGGAATGGGGAAGTGGATGTGGTGAGTTCAATCACATGTCTGGGAAAAGTCAATAGTGAAGACAGAGTCTCACAATTTTTTGTCATAATGGAGAGATGAAAACACAGGTAGAGGATTTCAAACAACAGAGTGGATGGTGAGTTAAAAATGCTGAAATTCTT(/G)TCCTGGTGTCTAACTTAATGCAATGTGGTTTATCTCTTTGCTCTTTTCTCTACTATTCAAATTTAGGATAATAAAGATTAAATGTTTCTAAATCTTACTTTACAATATCAAGAAAAAAAGGTATGCTTTTGCCCACGGAAGGGCAAAGCAGAGCTATGAAAACCTGCTGAACACATTCTTTATTTTCAACACAGGTTCTTGTCTTTCCATCATGAAATGCACATTTTATTTGTACTGTATTTGGGTGACCACAAGTCAACAACAAGATAATTCACAAGACCCTTGCCTTAGATGTGTCGGCAAT NGG 50 4 30 8 50 0 100 5 15
    240313225048_7d2d CCACCTGCGGGATGGGCCTGGTGCTGGGCGGTAAGGACACGGACCTGGAAGGAGCGCGCGCgagggagggaggctgggagtcagaatcgggaaagggaggtgcggggcggcgagggagcgaaggaggagaggag(/T)gaaggagcgggaggggTGCTGGCGGGGGTGCGTAGTGGGTGGAGAAAGCCGCTAGAGCAAATTTGGGGCCGGACCAGGCAGCACTCGGCTTTTAACCTGGGCAGTGAAGGCGGGGGAAAGAGCAAAAGGAAGGGGTGGTGTGCGGAGTAGGGGTGGGTGGGGGGAATTGGAAGCAAATGACATCACAGCAGGTCAGAGAAAAAGGGTTGAGCGGCAGGCACCCAGA NGG 20 8 17 8 40 0 100 5 10
    240313224906_774k tcagtcattaaatgcttgggctctggccacagattgtttaggtttaaatcccagtttcctcttttattattaattgtgcaacttgcttgggaaaacatgaaacttg(/C)tttttcctcaggttcattatctgtaatatatagtgaatgaagaagtttcctgtcccatgaaggtgttgtaaagattaaaaaaggcaaattaggctgtgtatttgtcataataattggcatatatggtaagtgaccaacaaccataaggtattatAAAATTGTTATAAAATGATATGAGCTATCATTGAGCAGCATGAAAGAAGAGCTTCACTGTTTCACCTACTATCACC NGG 20 8 17 8 40 0 100 5 10
    240313173223_j7up CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240313173222_n9yi CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10