Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240312014826_s7bh CGGGTTAGTTTACATTGTTCCTTGTCATTATCGCTAAAACACTCCAAAGCCTTCCTTAAAAATGCGCACTGGGCTAAAAAGGATAGACAAGGAACACATCCTGGGCCGGTAATTACGCAAAGCATTATCTCCTCTTACCTCCTTGCAGAtttttttttCTCTTTCAGTACGTGTCCTAAGATTTCTGTGCCACCCTTGGAGTTCACTCACCTAAACCTGAAACTAATAAAGCTTGGTTCTTTTCTCCGACACGCAAAGGAAGCGCTAAGGTAAATGCATCAGACCCACACTGCCGCGGAACTTTTCGGCTCTCTAAGGCTGTATTTTGATATACGAAAGGCACATTTTCCTTCCCTTTTCAAAATGCACCTTGCAAACGTAACAGGAACCCGACTAGGATCATCGGGAAAAGGAGGAGGAGGAGGAAGGCAGGCTCCGGGGAAGCTGGTGGCAGC(/G)GGGTCCTGGGTCTGGCGGACCCTGACGCGAAGGAGGGTCTAGGAAGCTCTCCGGGGAGCCGGTTCTCCCGCCGGTGGCTTCTTCTGTCCTCCAGCGTTGCCAACTGGACCTAAAGAGAGGCCGCGACTGTCGCCCACCTGCGGGATGGGCCTGGTGCTGGGCGGTAAGGACACGGACCTGGAAGGAGCGCGCGCgagggagggaggctgggagtcagaatcgggaaagggaggtgcggggcggcgagggagcgaaggaggagaggaggaaggagcgggaggggTGCTGGCGGGGGTGCGTAGTGGGTGGAGAAAGCCGCTAGAGCAAATTTGGGGCCGGACCAGGCAGCACTCGGCTTTTAACCTGGGCAGTGAAGGCGGGGGAAAGAGCAAAAGGAAGGGGTGGTGTGCGGAGTAGGGGTGGGTGGGGGGAATTGGAAGCAAATGACATCACAGCAGGTCAGAGAAAAAGGGTTGAGCGGCAGGCACCCAGA NGG 40 8 30 7 40 0 100 5 10
    240312014625_2lpj ATTTTCACTTGCTTGTTGATAACAAAGAAGAACTAATTATTAATTTATTTCAAAATGCATGTATTATATTTGATGGGCCACACTAACAGTTATAAACCAAACAACAGATTGGGAATGGGGAAGTGGATGTGGTGAGTTCAATCACATGTCTGGGAAAAGTCAATAGTGAAGACAGAGTCTCACAATTTTTTGTCATAATGGAGAGATGAAAACACAGGTAGAGGATTTCAAACAACAGAGTGGATGGTGAGTTAAAAATGCTGAAATTCTTTCCTGGTGTCTAACTTAATGCAATGTGGTTTATCTCTTTGCTCTTTTCTCTACTATTCAAATTTAGGATAATAAAGATTAAATGTTTCTAAATCTTACTTTACAATATCAAGAAAAAAAGGTATGCTTTTGCCCACGGAAGGGCAAAGCAGAGCTATGAAAACCTGCTGAACACATTCTTTATTTTCAACACAGGTTCTTGTCTTTCCATCATGAAATGCACATTTTATTTGTACTGTATTTGGGTGACCACAAGTCAACAACAAGATAATTCACAAGACCCTTGCCTTAGATGTGTCGGCAATAAAGTAATCAGGCCAAAATTTTTACTTTCCTTTGAATTTTTCAATTCAAACACAATGTATGCTTGCTTTTACACAGTAGGGTTCAGGGATTAGAGGGTTGGCTCTTTAAAAACCGTCAGAGACACAGGCAATCCTACACAAAATTCTCAGAAGGAAGGCGCCTACGCCTGGGAATGCCCAGATGCCCCTCAGAGAGTTGAAGATGGCGTTTCTCTGA(/T)GTCAGGTCAAAGTTAACACATTACCTTCGCTTCAAAGACTGCTTGGCTTCCTTTCGGTGGATTAGTCAAGATGTTTTGCTGACTGAGACTAGGAAATCTATAGGAGGGCGGGTTAGTTTACATTGTTCCTTGTCATTATCGCTAAAACACTCCAAAGCCTTCCTTAAAAATGCGCACTGGGCTAAAAAGGATAGACAAGGAACACATCCTGGGCCGGTAATTACGCAAAGCATTATCTCCTCTTACCTCCTTGCAGAtttttttttCTCTTTCAGTACGTGTCCTAAGATTTCTGTGCCACCCTTGGAGTTCACTCACCTAAACCTGAAACTAATAAAGCTTGGTTCTTTTCTCCGACACGCAAAGGAAGCGCTAAGGTAAATGCATCAGACCCACACTGCCGCGGAACTTTTCGGCTCTCTAAGGCTGTATTTTGATATACGAAAGGCACATTTTCCTTCCCTTTTCAAAATGCACCTTGCAAACGTAACAGGAACCCGACTAGGATCATCGGGAAA NGG 40 8 30 7 40 0 100 5 10
    240312014603_6m0j ATTTTCACTTGCTTGTTGATAACAAAGAAGAACTAATTATTAATTTATTTCAAAATGCATGTATTATATTTGATGGGCCACACTAACAGTTATAAACCAAACAACAGATTGGGAATGGGGAAGTGGATGTGGTGAGTTCAATCACATGTCTGGGAAAAGTCAATAGTGAAGACAGAGTCTCACAATTTTTTGTCATAATGGAGAGATGAAAACACAGGTAGAGGATTTCAAACAACAGAGTGGATGGTGAGTTAAAAATGCTGAAATTCTTTCCTGGTGTCTAACTTAATGCAATGTGGTTTATCTCTTTGCTCTTTTCTCTACTATTCAAATTTAGGATAATAAAGATTAAATGTTTCTAAATCTTACTTTACAATATCAAGAAAAAAAGGTATGCTTTTGCCCACGGAAGGGCAAAGCAGAGCTATGAAAACCTGCTGAACACATTCTTTATTTTCAACACAGGTTCTTGTCTTTCCATCATGAAATGCACATTTTATTTGTACTGTATTTGGGTGACCACAAGTCAACAACAAGATAATTCACAAGACCCTTGCCTTAGATGTGTCGGCAATAAAGTAATCAGGCCAAAATTTTTACTTTCCTTTGAATTTTTCAATTCAAACACAATGTATGCTTGCTTTTACACAGTAGGGTTCAGGGATTAGAGGGTTGGCTCTTTAAAAACCGTCAGAGACACAGGCAATCCTACACAAAATTCTCAGAAGGAAGGCGCCTACGCCTGGGAATGCCCAGATGCCCCTCAGAGAGTTGAAGATGGCGTTTCTCTGA(/T)GTCAGGTCAAAGTTAACACATTACCTTCGCTTCAAAGACTGCTTGGCTTCCTTTCGGTGGATTAGTCAAGATGTTTTGCTGACTGAGACTAGGAAATCTATAGGAGGGCGGGTTAGTTTACATTGTTCCTTGTCATTATCGCTAAAACACTCCAAAGCCTTCCTTAAAAATGCGCACTGGGCTAAAAAGGATAGACAAGGAACACATCCTGGGCCGGTAATTACGCAAAGCATTATCTCCTCTTACCTCCTTGCAGAtttttttttCTCTTTCAGTACGTGTCCTAAGATTTCTGTGCCACCCTTGGAGTTCACTCACCTAAACCTGAAACTAATAAAGCTTGGTTCTTTTCTCCGACACGCAAAGGAAGCGCTAAGGTAAATGCATCAGACCCACACTGCCGCGGAACTTTTCGGCTCTCTAAGGCTGTATTTTGATATACGAAAGGCACATTTTCCTTCCCTTTTCAAAATGCACCTTGCAAACGTAACAGGAACCCGACTAGGATCATCGGGAAA NGG 20 8 30 7 40 0 100 5 10
    240312014434_zaod ATTTTCACTTGCTTGTTGATAACAAAGAAGAACTAATTATTAATTTATTTCAAAATGCATGTATTATATTTGATGGGCCACACTAACAGTTATAAACCAAACAACAGATTGGGAATGGGGAAGTGGATGTGGTGAGTTCAATCACATGTCTGGGAAAAGTCAATAGTGAAGACAGAGTCTCACAATTTTTTGTCATAATGGAGAGATGAAAACACAGGTAGAGGATTTCAAACAACAGAGTGGATGGTGAGTTAAAAATGCTGAAATTCTTTCCTGGTGTCTAACTTAATGCAATGTGGTTTATCTCTTTGCTCTTTTCTCTACTATTCAAATTTAGGATAATAAAGATTAAATGTTTCTAAATCTTACTTTACAATATCAAGAAAAAAAGGTATGCTTTTGCCCACGGAAGGGCAAAGCAGAGCTATGAAAACCTGCTGAACACATTCTTTATTTTCAACACAGGTTCTTGTCTTTCCATCATGAAATGCACATTTTATTTGTACTGTATTTGGGTGACCACAAGTCAACAACAAGATAATTCACAAGACCCTTGCCTTAGATGTGTCGGCAATAAAGTAATCAGGCCAAAATTTTTACTTTCCTTTGAATTTTTCAATTCAAACACAATGTATGCTTGCTTTTACACAGTAGGGTTCAGGGATTAGAGGGTTGGCTCTTTAAAAACCGTCAGAGACACAGGCAATCCTACACAAAATTCTCAGAAGGAAGGCGCCTACGCCTGGGAATGCCCAGATGCCCCTCAGAGAGTTGAAGATGGCGTTTCTCTGA(/T)GTCAGGTCAAAGTTAACACATTACCTTCGCTTCAAAGACTGCTTGGCTTCCTTTCGGTGGATTAGTCAAGATGTTTTGCTGACTGAGACTAGGAAATCTATAGGAGGGCGGGTTAGTTTACATTGTTCCTTGTCATTATCGCTAAAACACTCCAAAGCCTTCCTTAAAAATGCGCACTGGGCTAAAAAGGATAGACAAGGAACACATCCTGGGCCGGTAATTACGCAAAGCATTATCTCCTCTTACCTCCTTGCAGAtttttttttCTCTTTCAGTACGTGTCCTAAGATTTCTGTGCCACCCTTGGAGTTCACTCACCTAAACCTGAAACTAATAAAGCTTGGTTCTTTTCTCCGACACGCAAAGGAAGCGCTAAGGTAAATGCATCAGACCCACACTGCCGCGGAACTTTTCGGCTCTCTAAGGCTGTATTTTGATATACGAAAGGCACATTTTCCTTCCCTTTTCAAAATGCACCTTGCAAACGTAACAGGAACCCGACTAGGATCATCGGGAAA NGG 20 8 17 8 40 0 100 5 10
    240312013956_72k4 tatAAAATTGTTATAAAATGATATGAGCTATCATTGAGCAGCATGAAAGAAGAGCTTCACTGTTTCACCTACTATCACCCTGGCCCATTAATCTCTTTCCTGTTCCTGACATTTCAGAGATACGTTTAGGATTTCAATCATGACCTTAAGCCACATTTGAACAATTTTCTGGTGGATAAGTCCTCATTCCCACATTATGTATGTACCTAGATGCAAATCCTGAATATCATGTCGCAATTAGTGCATCTGGACATGCTTGCTAACTGTGTTAAAGCTCTGAATAATGGTAAAGTTTTATTTCTACCAAAACAAATTTGGGCTGTAATGTTTTATGATAAAAATCTGTGGTCTTCCTATGTACATGTGTGTGTACATGCTTAAAATGCAATGTTATAGTTAAATGTAATTCATTAAAAGTATGTAACTCCAGTGGCTACTTAGTTTGGCTACTTGGTTTGTAGATTTCTGCTTTCCTGTTTCATTGTTAAACAGGTCTAGAAGTTATTATTTCATGAAACTAATGTGAGGAAAAAGACTATGTTGATATATAAGTGACATTATATAAATACATGAGGGATGATTTGATTagaagcagtattacacagtgataggagtaatggtttagaactagactcaggtttgaatcttagctctatcattataggcatttacttaacttttcttgtttgcttaactgaaaactgaagataataacacctatttacatggttgttataagggttatatgaataatgtctggcaaatagtaagaactcaagtaactgttTCACTCTTTCCAGAAGGA(/T)GATTGGCTGAAAAATATTTGGAGTCTCCTCCAGCCATATTCCTTGGTCAGCTTCTATGATCCTCTTTGGAGCTTAATTCTTAATCCCTTTATTTTCACTTGCTTGTTGATAACAAAGAAGAACTAATTATTAATTTATTTCAAAATGCATGTATTATATTTGATGGGCCACACTAACAGTTATAAACCAAACAACAGATTGGGAATGGGGAAGTGGATGTGGTGAGTTCAATCACATGTCTGGGAAAAGTCAATAGTGAAGACAGAGTCTCACAATTTTTTGTCATAATGGAGAGATGAAAACACAGGTAGAGGATTTCAAACAACAGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240312013755_xy7r TTATCTCCTCTTACCTCCTTGCAGAtttttttttCTCTTTCAGTACGTGTCCTAAGATTTCTGTGCCACCCTTGGAGTTCACTCACCTAAACCTGAAACTAATAAAGCTTGGTTCTTTTCTCCGACACGCAAAGGAAGCGCTAAGGTAAATGCATCAGACCCACACTGCCGCGGAACTTTTCGGCTCTCTAAGGCTGTATTTTGATATACGAAAGGCACATTTTCCTTCCCTTTTCAAAATGCACCTTGCAAACGTAACAGGAACCCGACTAGGATCATCGGGAAAAGGAGGAGGAGGAGGAAGGCAGGCTCCGGGGAAGCTGGTGGCAGCGGG(/T)TCCTGGGTCTGGCGGACCCTGACGCGAAGGAGGGTCTAGGAAGCTCTCCGGGGAGCCGGTTCTCCCGCCGGTGGCTTCTTCTGTCCTCCAGCGTTGCCAACTGGACCTAAAGAGAGGCCGCGACTGTCGCCCACCTGCGGGATGGGCCTGGTGCTGGGCGGTAAGGACACGGACCTGGAAGGAGCGCGCGCgagggagggaggctgggagtcagaatcgggaaagggaggtgcggggcggcgagggagcgaaggaggagaggaggaaggagcgggaggggTGCTGGCGGGGGTGCGTAGTGGGTGGAGAAAGCCGCTAGAGCAAATTTGGGGCCGGACCAGGCAGCACTCGGCTTTTAACCTGGGCAGTGAAGGCGGGGGAAAGAGCAAAAGGAAGGGGTGGTGTGCGGAGTAGGGGTGGGTGGGGGGAATTGGAAGCAAATGACATCACAGCAGGTCAGAGAAAAAGGGTTGAGCGGCAGGCACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240312013551_hiwk TGGCAGCGGGTCCTGGGTCTGGCGGACCCTGACGCGAAGGAGGGTCTAGGAAGCTCTCCGGGGAGCCGGTTCTCCCGCCGGTGGCTTCTTCTGTCCTCCAGCGTTGCCAACTGGACCTAAAGAGAGGCCGCGACTGTCGCCCACCTGCGGGATGGGCCTGGTGCTGGGCGGTAAGGACACGGACCTGGAAGGAGCGCGCGCgagggagggaggctgggagtcagaatcgggaaagggaggtgcggggcggcgagggagcgaaggaggagaggaggaaggagcgggaggggTGCTGGCGGGGGTGCGTAGTGGGTGGAGAAAGCCGCTAGAGCAAATTTGGGGCCGGACCAGGCAGCACTCGGCTTTTAACCTGGGCAGTGAAGGCGGGGGAAAGAGCAAAAGGAAGGGGTGGTGTGCGGAGTAGGGGTGGGTGGGGGGA(/C)ATTGGAAGCAAATGACATCACAGCAGGTCAGAGAAAAAGGGTTGAGCGGCAGGCACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240312013346_l820 CATAATGGAGAGATGAAAACACAGGTAGAGGATTTCAAACAACAGAGTGGATGGTGAGTTAAAAATGCTGAAATTCTTTCCTGGTGTCTAACTTAATGCAATGTGGTTTATCTCTTTGCTCTTTTCTCTACTATTCAAATTTAGGATAATAAAGATTAAATGTTTCTAAATCTTACTTTACAATATCAAGAAAAAAAGGTATGCTTTTGCCCACGGAAGGGCAAAGCAGAGCTATGAAAACCTGCTGAACACATTCTTTATTTTCAACACAGGTTCTTGTCTTTCCATCATGAAATGCACATTTTATTTGTACTGTATTTGGGTGACCACAAGTCAACAACAAGATAATTCACAAGACCCTTGCCTTAGATGTGTCGGCAATAAAGTAATCAGGCCAAAATTTTTACTTTCCTTTGAATTTTTCAATTCAAACACAATGTATGCTTGCTTTTACACAGTAGGGTTCAGGGATTAGAGGGTTGGCTCTTTAAAAACCGTCAGAGACACAGGCAATCCTACACAAAATTCTCAGAAGGAAGGCGCCTACGCCTGGGAATGCCCAGATGCCCCTCAGAGAGTTGAAGATGGCGTTTCTCTGAGTCAGGTCAAAGTTAACACATTACCTTCGCTTCAAAGACTGCTTGGCTTCCTTTCGGTGGATTAGTCAAGATGTTTTGCTGACTGAGACTAGGAAATCTATAGGAGGGCGGGTTAGTTTACATTGTTCCTTGTCATTATCGCTAAAACACTCCAAAGCCTTCCTTAAAAATGCGCACTGGGCTAAAAAGGATAGACAAGGAACACATCCTGGGCCGGTAATTACGCAAAGCATTATCTCCTCTTACCTCCTTGCAGAtttttttttCTCTTTCAGTACGTGTCCTAAGATTTCTGTGCCACCCTTGGAGTTCACTCACCTAAACCTGAAACTAATAAAGCTTGGTTCTTTTCTCCGACACGCAAAGGAAGCGCTAAGGTAAATGCATCAGACCCACACTGCCGCGGAACTTTTCGGCTCTCTAAGGCTGTATTTTGATATACGAAAGGCACATTTTCCTTCCCTTTTCAAAATGCACCTTGCAAACGTAACAGGAACCCGACTAGGATCATCGGGAAAAGGAGGAGGAGGAGGAAGGCAGGCTCCGGGGAAGCTGGTGGCAGC(/G)GGGTCCTGGGTCTGGCGGACCCTGACGCGAAGGAGGGTCTAGGAAGCTCTCCGGGGAGCCGGTTCTCCCGCCGGTGGCTTCTTCTGTCCTCCAGCGTTGCCAACTGGACCTAAAGAGAGGCCGCGACTGTCGCCCACCTGCGGGATGGGCCTGGTGCTGGGCGGTAAGGACACGGACCTGGAAGGAGCGCGCGCgagggagggaggctgggagtcagaatcgggaaagggaggtgcggggcggcgagggagcgaaggaggagaggaggaaggagcgggaggggTGCTGGCGGGGGTGCGTAGTGGGTGGAGAAAGCCGCTAGAGCAAATTTGGGGCCGGACCAGGCAGCACTCGGCTTTTAACCTGGGCAGTGAAGGCGGGGGAAAGAGCAAAAGGAAGGGGTGGTGTGCGGAGTAGGGGTGGGTGGGGGGAATTGGAAGCAAATGACATCACAGCAGGTCAGAGAAAAAGGGTTGAGCGGCAGGCACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240312001918_qarr ccggcctcgccacgcccctacctcaccacgccccccgcatcgccacgccccccgcatcgccacgcctcccttaccatgcagtcccgccccgtcccttcctcgtcccgcctcgccgcgacacttcacacacagcttcgcctcaccccattacagtctcaccacgccccgtcccCTCTCCGTTGAGCCCCGCGCCTTCGCCCGGGTGGGGCGCTGCGCTGTCAGCGGCCTTGCTGTGTGAGGCAGAACCTGCGGGGGCAGGGGCGGGCTGGTTCCCTGGCCAGCCATTGGCAGAGTCCGCAGGCTAGGGCTGTCAATCATGCTggccggcgtggccccgcctccgccggcgcggccccgcctccgccggcgcAGCGTCTGGGACGCAAGGCGCCGTGGGGGCTGCCGGGACGGGTCCAAGATGGACGGCCGCTCAGGTTCTGCTTTTACCTGCGGCCCAGAGCCCCATTCATTGCCCCGGTGCTGAGCGGCGCCGCGAGTCGGCCCGAGGCCTCCGGGGACTGCCGTGCCGGGCGGGAGACCGCCATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGTCCCTCAAGT(+ctc)CCTTCcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcaacagccgccaccgccgccgccgccgccgccgcctcctcagcttcctcagccgccgccgcaggcacagccgctgctgcctcagccgcagccgcccccgccgccgcccccgccgccacccggcccgGCTGTGGCTGAGGAGCCGCTGCACCGACCGTGAGTTTGGGCCCGCTGCAGCTCCCTGTCCCGGCGGGTCCCAGGCTACGGCGGGGATGGCGGTAACCCTGCAGCCTGCGGGCCGGCGACACGAACCCCCGGCCCCGCAGAGACAGAGTGACCCAGCAACCCAGAGCCCATGAGGGACACCCGCCCCCTCCTGGGGCGAGGCCTTCCCCCACTTCAGCCCCGCTCCCTCACTTGGG NG 10 8 17 8 24 0 100 5 10
    240312001854_54sd ccggcctcgccacgcccctacctcaccacgccccccgcatcgccacgccccccgcatcgccacgcctcccttaccatgcagtcccgccccgtcccttcctcgtcccgcctcgccgcgacacttcacacacagcttcgcctcaccccattacagtctcaccacgccccgtcccCTCTCCGTTGAGCCCCGCGCCTTCGCCCGGGTGGGGCGCTGCGCTGTCAGCGGCCTTGCTGTGTGAGGCAGAACCTGCGGGGGCAGGGGCGGGCTGGTTCCCTGGCCAGCCATTGGCAGAGTCCGCAGGCTAGGGCTGTCAATCATGCTggccggcgtggccccgcctccgccggcgcggccccgcctccgccggcgcAGCGTCTGGGACGCAAGGCGCCGTGGGGGCTGCCGGGACGGGTCCAAGATGGACGGCCGCTCAGGTTCTGCTTTTACCTGCGGCCCAGAGCCCCATTCATTGCCCCGGTGCTGAGCGGCGCCGCGAGTCGGCCCGAGGCCTCCGGGGACTGCCGTGCCGGGCGGGAGACCGCCATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGTCCCTCAAGT(+ctc)CCTTCcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcagcaacagccgccaccgccgccgccgccgccgccgcctcctcagcttcctcagccgccgccgcaggcacagccgctgctgcctcagccgcagccgcccccgccgccgcccccgccgccacccggcccgGCTGTGGCTGAGGAGCCGCTGCACCGACCGTGAGTTTGGGCCCGCTGCAGCTCCCTGTCCCGGCGGGTCCCAGGCTACGGCGGGGATGGCGGTAACCCTGCAGCCTGCGGGCCGGCGACACGAACCCCCGGCCCCGCAGAGACAGAGTGACCCAGCAACCCAGAGCCCATGAGGGACACCCGCCCCCTCCTGGGGCGAGGCCTTCCCCCACTTCAGCCCCGCTCCCTCACTTGGG NG 10 8 17 8 24 0 100 5 10