Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240309052250_cmmb CACGCAAAGGAAGCGCTAAGGTAAATGCATCAGACCCACACTGCCGCGGAACTTTTCGGCTCTCTAAGGCTGTATTTTGATATACGAAAGGCACATTTTCCTTCCCTTTTCAAAATGCACCTTGCAAACGTAACAGGAACCCGACTAGGATCATCGGGAAAAGGAGGAGGAGGAGGAAGGCAGGCTCCGGGGAAGCTGGTGGCAGC(/G)GGGTCCTGGGTCTGGCGGACCCTGACGCGAAGGAGGGTCTAGGAAGCTCTCCGGGGAGCCGGTTCTCCCGCCGGTGGCTTCTTCTGTCCTCCAGCGTTGCCAACTGGACCTAAAGAGAGGCCGCGACTGTCGCCCACCTGCGGGATGGGCCTGGTGCTGGGCGGTAAGGACACGGACCTGGAAGGAGCGCGCGCgagggagggaggctgggagtcagaatcgggaaagggaggtgcggggcggcgagggagcgaaggaggagaggaggaaggagcgggaggggTGCTGGCGGGGGTGCGTAGTGGGTGGAGAAAGCCGCTAGAGCAAATTTGGGGCCGGA NGG 20 8 20 8 40 0 100 5 10
    240309050932_nxrh AAGCAGAGCTATGAAAACCTGCTGAACACATTCTTTATTTTCAACACAGGTTCTTGTCTTTCCATCATGAAATGCACATTTTATTTGTACTGTATTTGGGTGACCACAAGTCAACAACAAGATAATTCACAAGACCCTTGCCTTAGATGTGTCGGCAATAAAGTAATCAGGCCAAAATTTTTACTTTCCTTTGAATTTTTCAATTCAAACACAATGTATGCTTGCTTTTACACAGTAGGGTTCAGGGATTAGAGGGTTGGCTCTTTAAAAACCGTCAGAGACACAGGCAATCCTACACAAAATTCTCAGAAGGAAGGCGCCTACGCCTGGGAATGCCCAGATGCCCCTCAGAGAGTTGAAGATGGCGTTTCTCTGA(/T)GTCAGGTCAAAGTTAACACATTACCTTCGCTTCAAAGACTGCTTGGCTTCCTTTCGGTGGATTAGTCAAGATGTTTTGCTGACTGAGACTAGGAAATCTATAGGAGGGCGGGTTAGTTTACATTGTTCCTTGTCATTATCGCTAAAACACTCCAAAGCCTTCCTTAAAAATGCGCACTGGGCTAAAAAGGATAGACAAGGAACACATCCTGGGCCGGTAATTACGCAAAGCATTATCTCCTCTTACCTCCTTGCAGAtttttttttCTCTTTCAGTACGTGTCCTAAGATTTCTGTGCCACCCTTGGAGTTCACTCACCTAAACCTGAAACTAATAAAGCTTGGTTCTTTTCTCCGACACGCAAAGGAAGC NGG 40 7 20 8 40 0 100 5 10
    240309050310_v12u AATCTATAGGAGGGCGGGTTAGTTTACATTGTTCCTTGTCATTATCGCTAAAACACTCCAAAGCCTTCCTTAAAAATGCGCACTGGGCTAAAAAGGATAGACAAGGAACACATCCTGGGCCGGTAATTACGCAAAGCATTATCTCCTCTTACCTCCTTGCAGAtttttttttCTCTTTCAGTACGTGTCCTAAGATTTCTGTGCCACCCTTGGAGTTCACTCACCTAAACCTGAAACTAATAAAGCTTGGTTCTTTTCTCCGACACGCAAAGGAAGCGCTAAGGTAAATGCATCAGACCCACACTGCCGCGGAACTTTTCGGCTCTCTAAGGCTGTATTTTGATATACGAAAGGCACATTTTCCTTCCCTTTTCAAAATGCACCTTGCAAACGTAACAGGAACCCGACTAGGATCATCGGGAAAAGGAGGAGGAGGAGGAAGGCAGGCTCCGGGGAAGCTGGTGGCAGC(/G)GGGTCCTGGGTCTGGCGGACCCTGACGCGAAGGAGGGTCTAGGAAGCTCTCCGGGGAGCCGGTTCTCCCGCCGGTGGCTTCTTCTGTCCTCCAGCGTTGCCAACTGGACCTAAAGAGAGGCCGCGACTGTCGCCCACCTGCGGGATGGGCCTGGTGCTGGGCGGTAAGGACACGGACCTGGAAGGAGCGCGCGCgagggagggaggctgggagtcagaatcgggaaagggaggtgcggggcggcgagggagcgaaggaggagaggaggaaggagcgggaggggTGCTGGCG NGG 10 8 17 8 24 0 100 5 10
    240309045940_yexp agcgaaggaggagaggaggaaggagcgggaggggTGCTGGCGGGGGTGCGTAGTGGGTGGAGAAAGCCGCTAGAGCAAATTTGGGGCCGGACCAGGCAGCACTCGGCTTTTAACCTGGGCAGTGAAGGCGGGGGAAAGAGCAAAAGGAAGGGGTGGTGTGCGGAGTAGGGGTGGGTGGGGGGA(/C)ATTGGAAGCAAATGACATCACAGCAGGTCAGAGAAAAAGGGTTGAGCGGCAGGCACCCAGA NGG 10 7 20 8 40 0 100 5 10
    240309044619_hspq GGCAGTGAAGGCGGGGGAAAGAGCAAAAGGAAGGGGTGGTGTGCGGAGTAGGGGTGGGTGGGGGGA(/C)ATTGGAAGCAAATGACATCACAGCAGGTCAGAGAAAAAGGGTTGAGCGGCAGGCACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240308200811_5xvp cgggcgcggcgtggactccgtccccctggctggaccatg(-g)tgaacacccggaagagctctctccgccttctcgggtccaag NGG 10 8 17 8 24 0 100 5 10
    240308195731_pfax cgggcgcggcgtggactccgtccccctggctggaccat(/a)ggtgaacacccggaagagctctctccgccttctcgggtccaag NGG 10 8 17 8 24 0 100 5 10
    240308195632_hc48 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240308174934_lvuk tggactccgtccccctggctggaccat(/a)ggtgaacacccgg NGG 10 8 17 8 24 0 100 5 10
    240308164617_qgiz CCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAACTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGTTCCTGAAGCTC NG 20 8 16 7 15 37 97 5 10