Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240301111906_hvam CCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAACTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGTTCCTGAAGCTC NG 10 8 17 8 24 0 100 5 10
    240301111905_aj3l CCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAACTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGTTCCTGAAGCTC NG 10 8 17 8 24 0 100 5 10
    240301111804_4qaa CCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAACTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGTTCCTGAAGCTC NG 10 8 17 8 24 0 100 5 10
    240229152355_hj0k AAGTGCTGGGATTACAGGCATGAACCACCACACCTGGCCAGCAGTCATCTCTTTACCAACTTTGCTACTTGCCTTTTCCTTCCAGAGGCTGACAAGTCTGCCTACCTCATGGGGCTGAACTCAGCCGACCTGCTCAAGGGGCTGTGCCACCCTCG(/A)GGTGAAAGTGGGCAATGAGTACGTCACCAAGGGGCAGAATGTCCAGCAGGTGGGTCCATCTTCAGATGATAATGGGTGGGCAGGGTAGGGAGACTGGCATGGTGGGATGAGAGTTTTCAAGTTCACTCTTCCCAAC NGG 10 10 15 10 20 0 50 5 3
    240229152217_c8ql AAGTGCTGGGATTACAGGCATGAACCACCACACCTGGCCAGCAGTCATCTCTTTACCAACTTTGCTACTTGCCTTTTCCTTCCAGAGGCTGACAAGTCTGCCTACCTCATGGGGCTGAACTCAGCCGACCTGCTCAAGGGGCTGTGCCACCCTCG(/A)GGTGAAAGTGGGCAATGAGTACGTCACCAAGGGGCAGAATGTCCAGCAGGTGGGTCCATCTTCAGATGATAATGGGTGGGCAGGGTAGGGAGACTGGCATGGTGGGATGAGAGTTTTCAAGTTCACTCTTCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240229141100_o6gr CCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAACTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGTTCCTGAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240229125118_q5pa aataccatctgtttccggaacccaagttcgtcacgttgtgtccagtgcccccctgaaatgtccttatgtgattagagttctgggagcttccttattgaactctcaacctgcctctgactctgtcctgttttcagaaccccaacaagcacatctcccagacagaggtgatcatccggtttgctttccagacgtccatcacggtgctgtgcattgcctgcccctgctccctggggctggccacgcC(/T)cacggctgtcatggtgggcaccggggtggccgcgcagaacggcatcctcatcaagggaggcaagcccctggagatggcgcacaaggtcagcctgtagcacggctttccccatcctgagagatgaaagtagtatctgtttactatttcacattgagagaaaagcctgagagccactcaagacagcagtgttaattacatagaataggaagtcaagtataactgggaataacaacagt NGG 10 8 17 8 24 0 100 5 10
    240229125012_4exv CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240229024131_5qev TGCCTGCGAAGTGAAGGGTCTCCGCTGGTGGTCCTACCATACATGAAACATGGAGATCTTCGAAATTTCA(/T)TTCGAAATGAGACTCATGTAAGTTGACTGCCAAGCTTACTAACTGGCAAACTAGCTGTAAGCCAGCCATCCCTTCAAAATAGGCCTGCTCTGAGTCTTTAAAAAGCTAGT NGG 30 8 17 8 35 0 70 5 10
    240229023133_cz1z ACTGACATAGGAGAAGTTTCCCAATTTCTGACCGAGGGAATCATCATGAAAGATTTTAGTCATCCCAATGTCCTCTCGCTCCTGGGAATCTGCCTGCGAAGTGAAGGGTCTCCGCTGGTGGTCCTACCATACATGAAACATGGAGATCTTCGAAATTTCA(/T)TTCGAAATGAGACTCATGTAAGTTGACTGCCAAGCTTACTAACTGGCAAACTAGCTGTAAGCCAGCCATCCCTTCAAAATAGGCCTGCTCTGAGTCTTTAAAAAGCTAG NGG 30 8 17 8 35 0 100 5 10