Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240227060405_ia8q TTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTACG(/A)GCATTGTTCCACAATGCCACGC NGG 10 8 17 8 24 0 100 5 10
    240227001102_0f9c CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240227001006_8jov AGTCAATCATCCAGGTTAGGGAGCAGCAGGTGCAGGGCCCAGGG(/C)TGGACTGGGTGCTGCAGCCCCTCACCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240226235754_7tow ttagaagaggatgatggtgagcacg(+AAGGA)tgatggtgagcacgtgatggtgagcacgtgatggtgagcacgtgatggtgagcacgtgatggtgagccaaggaaa NGG 10 8 17 8 24 0 100 5 10
    240226234719_53ze ttagaagaggatgatggtgagcacg(+AAGGA)tgatggtgagcacgtgatggtgagcacgtgatggtgagcacgtgatggtgagcacgtgatggtgagccaaggaaa NGG 10 8 17 8 24 0 100 5 10
    240226150126_zr4r ccaccggcaagctgccGgtgccctggcccaccctcgtgaccaccctgaccC(/T)ATggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttc NGG 10 8 17 8 24 0 100 5 10
    240226144338_2xhy CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 20 17 17 8 24 0 100 5 10
    240226144303_wogg accggcaagctgccggtgccctggcccaccctcgtgaccaccctgaccC(/T)ATggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgact NGG 10 8 17 8 24 0 100 5 10
    240226135242_bsml ctgaccctgaagttcatctgcaccaccggcaagctgccGgtgccctggcccaccctcgtgaccaccctgaccC(/T)ATggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagc NGG 50 17 20 8 40 0 100 5 10
    240226135051_f0pk ctgaccctgaagttcatctgcaccaccggcaagctgccGgtgccctggcccaccctcgtgaccaccctgaccC(/T)ATggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagc NGG 10 8 17 8 24 0 100 5 10