Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240308134751_fkpj CCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAACTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGTTCCTGAAGCTC NG 20 8 16 7 15 0 97 5 10
    240308133510_26w3 CCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAACTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGTTCCTGAAGCTC NGG 20 8 16 7 15 0 97 5 10
    240308133003_0x1r CCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAACTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGTTCCTGAAGCTC NGG 20 8 16 7 15 0 97 5 10
    240308095747_wqg3 GGAATTACAGGTGTGAGCCACGGCTCCTGGCCAAGACCTAGAAGTTTTACGTCAAATTTTGTCTCTGCAGTTTTCTGAGGAATGAGTATATGTGCTCAAATAATAGCTAAGTATTAAGTCTGAAATATTCTAGAAAAACAGTCACAAAGTAGTTATGCCTAGGATGTTTTTGAATATTGGTACTAGAACAAATGGATTCCTACTAGATTTCCTTTTTACTTTCATATTCAGATGTAGGAAGAGTTGGAATTCAAAATGTGAAAAGCAGTCTTGAATCTCAGTAAATTCTTAAGAAATTCTTAATTCATTGAAGAGCCATTGGTCGTGTGTGTTTTTTGGGGAGTGTGTGTGTGTGTTGTTGTTGTTAAGAATTCGGGGATGAGTATGAGGCTATCATTGCTTCTACAAGGCAACTTAATGATAGTTTGCTGCTTGGCACAACAGGTGAAAGGGCCTTTCTAGAGCAGGGATCTTGGTTTCCCTTTTTCCCACCT(+CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC)AGTAGTATCATTCCATGTATTTACCAGGATATACATTGTTTAGTCCTGTCTTGGCTAAATAGGTAATGGTCCTTAAGGACATAAAATGGAAGGATATTGTGCAGAAGAATTACTAGTTCTCCGTAGTGTATTTTTTTACTGTATTCTCCTCATCAACAGATGGAAAATTTTTAAACTTAGAAGAAAAAACAAGTGTTTTTCTCCAAAAGATTGAGAACCACTGCTTTTAAGTTATTAATGGAGGTCTTCAATGGATTTGTTAAAAATCTGGAGCATGGATTTTTTTTTTTTAATTTTTTTGTTATTGGTTACTGAGTATTGGAAAAGGAAATACAGTATTCAAAAACAGTAATGTTCAGGATTTGGAACTATTTTACTCAGGAAAAAATAAGTTTATAAATAGACATTTTAATGAAAAGGAAAATAGCAAATTTATGGAAATCTGAGATAGTCTGTGTTTTGCTTCACTCTGTTTAGTGCTAGAATTCTGGACTTTGTTTGGGGTCAAACTCTGATATGACAGGAAAACTAACAGATGATTAAAAG NGG 10 8 17 8 82 0 100 5 10
    240308094333_5tte GGAATTACAGGTGTGAGCCACGGCTCCTGGCCAAGACCTAGAAGTTTTACGTCAAATTTTGTCTCTGCAGTTTTCTGAGGAATGAGTATATGTGCTCAAATAATAGCTAAGTATTAAGTCTGAAATATTCTAGAAAAACAGTCACAAAGTAGTTATGCCTAGGATGTTTTTGAATATTGGTACTAGAACAAATGGATTCCTACTAGATTTCCTTTTTACTTTCATATTCAGATGTAGGAAGAGTTGGAATTCAAAATGTGAAAAGCAGTCTTGAATCTCAGTAAATTCTTAAGAAATTCTTAATTCATTGAAGAGCCATTGGTCGTGTGTGTTTTTTGGGGAGTGTGTGTGTGTGTTGTTGTTGTTAAGAATTCGGGGATGAGTATGAGGCTATCATTGCTTCTACAAGGCAACTTAATGATAGTTTGCTGCTTGGCACAACAGGTGAAAGGGCCTTTCTAGAGCAGGGATCTTGGTTTCCCTTTTTCCCACCT(+CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC)AGTAGTATCATTCCATGTATTTACCAGGATATACATTGTTTAGTCCTGTCTTGGCTAAATAGGTAATGGTCCTTAAGGACATAAAATGGAAGGATATTGTGCAGAAGAATTACTAGTTCTCCGTAGTGTATTTTTTTACTGTATTCTCCTCATCAACAGATGGAAAATTTTTAAACTTAGAAGAAAAAACAAGTGTTTTTCTCCAAAAGATTGAGAACCACTGCTTTTAAGTTATTAATGGAGGTCTTCAATGGATTTGTTAAAAATCTGGAGCATGGATTTTTTTTTTTTAATTTTTTTGTTATTGGTTACTGAGTATTGGAAAAGGAAATACAGTATTCAAAAACAGTAATGTTCAGGATTTGGAACTATTTTACTCAGGAAAAAATAAGTTTATAAATAGACATTTTAATGAAAAGGAAAATAGCAAATTTATGGAAATCTGAGATAGTCTGTGTTTTGCTTCACTCTGTTTAGTGCTAGAATTCTGGACTTTGTTTGGGGTCAAACTCTGATATGACAGGAAAACTAACAGATGATTAAAAG NGG 10 8 17 8 82 0 100 5 10
    240308081550_7py1 tactggccttatctcacaggtaaaactgacgcacggaggaacaatataaattggggactagaaaggtgaagagccaaagttagaactcaggaccaacttattctgattttgtttttccaaactgcTTCTCCTCTTGGGAAGTGTAAGGAAGCTGCAGCACCAGGATCAGTGAAACGCACCAGACGGCCGCGTCAGAGCAGCTCAGGTTCTGGGAGAGGGTAGCGCAGGGTGGCCACTGAGAACCGGGCAGGTCACGCATCCCCCCCTTCCCTCCCACCCCCTGCCAAGCTCTCCCTCCCAGGA(+CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC)TCCTCTCTGGCTCCATCGTAAGCAAACCTTAGAGGTTCTGGCAAGGAGAGAGATGGCTCCAGGAAATGGGGGTGTGTCACCAGATAAGGAATCTGCCTAACAGGAGGTGGGGGTTAGACCCAATATCAGGAGACTAGGAAGGAGGAGGCCTAAGGATGGGGCTTTTCTGTCACCAATCCTGTCCCTAGTGGCCCCACTGTGGGGTGGAGGGGACAGATAAAAGTACCCAGAACCAGAGCCACATTAACCGGCCCTGGG NGG 10 8 17 8 82 0 100 5 100
    240308081311_vyn2 tactggccttatctcacaggtaaaactgacgcacggaggaacaatataaattggggactagaaaggtgaagagccaaagttagaactcaggaccaacttattctgattttgtttttccaaactgcTTCTCCTCTTGGGAAGTGTAAGGAAGCTGCAGCACCAGGATCAGTGAAACGCACCAGACGGCCGCGTCAGAGCAGCTCAGGTTCTGGGAGAGGGTAGCGCAGGGTGGCCACTGAGAACCGGGCAGGTCACGCATCCCCCCCTTCCCTCCCACCCCCTGCCAAGCTCTCCCTCCCAGGA(+CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC)TCCTCTCTGGCTCCATCGTAAGCAAACCTTAGAGGTTCTGGCAAGGAGAGAGATGGCTCCAGGAAATGGGGGTGTGTCACCAGATAAGGAATCTGCCTAACAGGAGGTGGGGGTTAGACCCAATATCAGGAGACTAGGAAGGAGGAGGCCTAAGGATGGGGCTTTTCTGTCACCAATCCTGTCCCTAGTGGCCCCACTGTGGGGTGGAGGGGACAGATAAAAGTACCCAGAACCAGAGCCACATTAACCGGCCCTGGG NGG 10 8 17 8 82 0 100 5 10
    240308080825_k9z7 AGGGAGTGGAGGAAGACGGAACCTGAAGGAGGCGGCAGGGAAGGATCTGGGCCAGCCGTAGAGGTGACCCAGGCCACAAGCTGCAGACAGAAAGCGGCACAGGCCCAGGGGAGAGAA(+CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC)TGCAGGTCAGAGAAAGCAGGACCTGCCTGGGAAGGGGAAACAGTGGGCCAGAGGCGGCGCAGAAGCCAGTAGAGCTCAAAGTGGTCCGGACTCAGGAGAGAGA NGG 10 8 17 8 82 0 100 5 10
    240307014928_iimm CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240306234552_pqyo CCGAGGAGGAGCTGATGGAAGTTGACGAGGCTGGCAGCGTGTACGCGGGTGTCCTCAGCTACG(/a)GGGTGGGCTTCTTCCTCTTCATCCTGGTGGTGGCGG NGG 10 8 17 8 24 0 100 5 10