Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240222004221_fnwd GAGCTCCACATGGAAACACACGGACTTGGTCTTCACTCTTTCTCTCCTCAACACAACAGGCCCATATTTTATTACT(/C)GTGGCAACACTCTGATGGCTGTCACTCTTGGCCAGTACAAAGCACACCTCTGGACTTGGACCAACTCCTGGGAGGAGTTCACACAG NGG 10 8 17 8 24 0 100 5 10
    240221050728_bhyy CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240220094735_tjxz TTGTGGCCTATGACAAGAAAATGGACACCAACAAGGACGACCCAGAGCTGATGCTGGGCCGCATGCGCTG(/A)CCTCGAGGCCTTGGGGGAATGGGGTCAACTC NGG 10 8 17 8 24 0 100 5 10
    240220094404_se5t CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240220094253_s0xt TTGTGGCCTATGACAAGAAAATGGACACCAACAAGGACGACCCAGAGCTGATGCTGGGCCGCATGCGCTG(/A)CCTCGAGGCCTTGGGGGAATGGGGTCAACTC NGG 10 8 17 8 24 0 100 5 10
    240220094145_c134 TTGTGGCCTATGACAAGAAAATGGACACCAACAAGGACGACCCAGAGCTGATGCTGGGCCGCATGCGCTG(/A)CCTCGAGGCCTTGGGGGAATGGGGTCAACTC NGG 10 8 17 8 24 0 100 5 10
    240219164114_2xc6 CCTTTTGATCAGTTCCATGACAGCCATCGCTACATAGAACATTATACTCTACAACCTGAAACAGGAGCACTCAATCTCATTGATCCAATACATGAGTTCAAAGCTCTCACAAACACAGAAACAGCCACGGAAGTGGACATTAAGATGAAATTCAGCAATGAAGTCTTCCGATTTGCATCAGCTTGTATGAATTCACGCAC(/A)CAATGGCACCATCCATTTTGGAGTCAAGGACAAACCCCATGGAGAAATTGTTGGTGTGAAAATCACCAGTAAGGCTGCCTTCATTGACCACTTCAATGTAATGATCAAAAAGTATTTTGAAGAAAGTGAGATCAATGAAGCCAAGAAGTGTATTCGGGAGCCAAGGTTTGTGGAAGTCCTTCTGCAGAACAATACACCAT NGG 10 8 17 8 24 0 100 5 10
    240219151751_lstw CCACTCTGCGTGCCCTGGGCTGTCTGGGGCTCCCACTACCAGTCCCACCTTCCTTGCCTTCCCCACAGGCTGAAGCTGTCCCCCAGCCCATCCTCGCGCGTCACCGTCTCACGAGCCACCTCGAGCAGCAGCGGCAGCT(/A)TGTCCGCCACCGGGCGCCTGGGCCGCAGTAAGCGGAAGCGGCTGGAGGTGGAGGAGCCCTTGGGCAGCGGCCCAAGCGTCCTGGGCACGGGCACGGGTGGCAGCGGTGGCTTCCACCTGGCCCAGCAGGCCTCGGCCTCGGGTAGCGTCAGCATCGAGGAGATCGACCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240219055747_clog CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240217230432_tdwe CCTTTTGATCAGTTCCATGACAGCCATCGCTACATAGAACATTATACTCTACAACCTGAAACAGGAGCACTCAATCTCATTGATCCAATACATGAGTTCAAAGCTCTCACAAACACAGAAACAGCCACGGAAGTGGACATTAAGATGAAATTCAGCAATGAAGTCTTCCGATTTGCATCAGCTTGTATGAATTCACGCAC(/A)CAATGGCACCATCCATTTTGGAGTCAAGGACAAACCCCATGGAGAAATTGTTGGTGTGAAAATCACCAGTAAGGCTGCCTTCATTGACCACTTCAATGTAATGATCAAAAAGTATTTTGAAGAAAGTGAGATCAATGAAGCCAAGAAGTGTATTCGGGAGCCAAGGTTTGTGGAAGTCCTTCTGCAGAACAATACACCAT NG 10 8 17 8 24 0 100 5 10