Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240214193200_z9gk TCTAAACTGGGCAAAACAAGCAAAAATCATAGAACCTGACAATTCTTATATCTCAGATACACTGGGTCAAGTCTACAAAAGTAAAATAAGATGGTGGATAG(/C)AGGAAAACGGAGGAAACGGGAACATTTCAGTTGATGATCTAATTGCTCTTTTGGATTTAGCAGAACATGCCTCAAGTGCATTCAAAGAATCTCAACAGCA NG 10 8 17 8 24 0 100 5 10
    240214192142_o3g2 TCTAAACTGGGCAAAACAAGCAAAAATCATAGAACCTGACAATTCTTATATCTCAGATACACTGGGTCAAGTCTACAAAAGTAAAATAAGATGGTGGATA(/C)GAGGAAAACGGAGGAAACGGGAACATTTCAGTTGATGATCTAATTGCTCTTTTGGATTTAGCAGAACATGCCTCAAGTGCATTCAAAGAATCTCAACAGC NGG 10 8 17 8 24 0 100 5 10
    240214190025_ih0m TCTAAACTGGGCAAAACAAGCAAAAATCATAGAACCTGACAATTCTTATATCTCAGATACACTGGGTCAAGTCTACAAAAGTAAAATAAGATGGTGGATA(/C)AGGAAAACGGAGGAAACGGGAACATTTCAGTTGATGATCTAATTGCTCTTTTGGATTTAGCAGAACATGCCTCAAGTGCATTCAAAGAATCTCAACAGCA NG 10 8 17 8 24 0 100 5 10
    240214181227_wi0y CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240214100010_sfqj acgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagggttcaggggagggaagaggttccctgctcacatgcggagacgtagaAgaAaaCcccggacctgctagcAGCCTACCTCATCTGCGAATGACAAGCTTAAGTGTCTCGATTACCACACCCCTCCCAACCCCTCAGGCCTCAATGGAC(-AA)AAGAGCTCTTGCTGCATCGTAGCCACCAGTACTCAGGGCAGTGTGCTCCCGGCCTGGTCTGCTagtaGATCTACCTTGGCGAGTTCGgtcgacagcaaaggagaagaacttttcactgg NGG 10 8 17 8 24 0 100 5 10
    240212181917_7fqa CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240212181658_9vrb CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240212175135_goj4 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240212174828_m5au CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240211190120_r4wb tggcgaaaaggtagctgtgaaagtgttcttcactacagaggaggccagctggttccgagagacagaaatatatca(/g)gacggtgttgatgaggcatgaaaacatcttgggtgagtataagtctgtattagcgatgttcagggtttcctagct NGG 10 8 17 8 24 0 100 5 10