Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240120070159_khrl ATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTCGGGTTCAAGGCCAGCCTT(+GACTACAAAGACGATGACGACAAG)AACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTA NGG 10 8 17 8 50 0 100 5 10
    240120070103_l920 ATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTCGGGTTCAAGGCCAG(+GACTACAAAGACGATGACGACAAG)CCTTAACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTA NGG 10 8 17 8 50 0 100 5 10
    240120070026_l706 ATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTCGGGTTCAAGGCCAG(+GACTACAAAGACGATGACGACAAG)CCTTAACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTA NGG 10 8 17 8 50 0 100 5 10
    240120065615_bo7x GATGTCCAAGAGGGGGAAAAAGACAGCACGGGTAACGTAACTTATGCAAAGCCTATTGATACAGGTAGTTGGGACATGATATCCCAACATAGAGAAAATATTAAAAGACCTGTGAATGTGGGTGCTGCGGCAACGAAGCAAAAAGTCATGGATTTGAATTATAAAATGGATGAT(+GACTACAAAGACGATGACGACAAG)GGGAATAGAGAAAATAGTGAAGCTTAATATCAGTTTATAATGGTTGAGCGTTTGTTCCTTGGATTATGTAAATAAGATTTTTATGAGACGTTATTTTATCTTAAGAAATACATTTATATTTTTGTATGTCTGTCTTGTGTTTTAGTCAAGCCC NGG 10 8 17 8 50 0 100 5 10
    240120065015_7ti2 CTATTATTTGAAGTACTCATAAACGTAAGTAACAGTACATTTTTTTTTCATATTACATTATGTGAATACGTCAATCATAAATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTCGGGTTCAAG(+GACTACAAAGACGATGACGACAAG)GCCAGCCTTAACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTAAAACCCCGCTGTTGAGAG NGG 10 8 17 8 50 0 100 5 10
    240120064740_1gyn CTATTATTTGAAGTACTCATAAACGTAAGTAACAGTACATTTTTTTTTCATATTACATTATGTGAATACGTCAATCATAAATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTC(+GACTACAAAGACGATGACGACAAG)GGGTTCAAGGCCAGCCTTAACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTAAAACCCCGCTGTTGAGAG NGG 10 8 17 8 50 0 100 5 10
    240120062016_6xz5 CTATTATTTGAAGTACTCATAAACGTAAGTAACAGTACATTTTTTTTTCATATTACATTATGTGAATACGTCAATCATAAATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTCGGGTTCAAG(+GACTACAAAGACGATGACGACAAG)GCCAGCCTTAACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTAAAACCCCGCTGTTGAGAG NGG 10 8 17 8 50 0 100 5 10
    240120061853_hibj CTATTATTTGAAGTACTCATAAACGTAAGTAACAGTACATTTTTTTTTCATATTACATTATGTGAATACGTCAATCATAAATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTCGGGTTC(+GACTACAAAGACGATGACGACAAG)AAGGCCAGCCTTAACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTAAAACCCCGCTGTTGAGAG NGG 10 8 17 8 50 0 100 5 10
    240120061633_q0cy CTATTATTTGAAGTACTCATAAACGTAAGTAACAGTACATTTTTTTTTCATATTACATTATGTGAATACGTCAATCATAAATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTC(+GACTACAAAGACGATGACGACAAG)GGGTTCAAGGCCAGCCTTAACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTAAAACCCCGCTGTTGAGAG NGG 10 8 17 8 50 0 100 5 10
    240120061457_2h7m CTATTATTTGAAGTACTCATAAACGTAAGTAACAGTACATTTTTTTTTCATATTACATTATGTGAATACGTCAATCATAAATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTCGGGTTCAA(+GACTACAAAGACGATGACGACAAG)GGCCAGCCTTAACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTAAAACCCCGCTGTTGAGAG NGG 10 8 17 8 50 0 100 5 10