Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240115180650_akwj gaaagaattcagattcagtgggatgaggttcaagaacagctgcagaacaggagat(/c)aacagttgaatgaaatgttaaaggattcaacacaatggctggaagctaaggaagaagccgaacaggtcataggacaggtcagaggcaagcttgactcatggaaagaaggtcctcacacagtagatgcaatccaaaagaagatcacagaaaccaag NGG 10 8 17 8 24 0 100 5 10
    240115111407_4bdo CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240114234338_p7sz gaaagaattcagattcagtgggatgaggttcaagaacagctgcagaacaggagat(/c)aacagttgaatgaaatgttaaaggattcaacacaatggctggaagctaaggaagaagccgaacaggtcataggacaggtcagaggcaagcttgactcatggaaagaaggtcctcacacagtagatgcaatccaaaagaagatcacagaaaccaag NGG 10 8 17 8 24 0 100 5 10
    240114223537_lmsr gaaagaattcagattcagtgggatgaggttcaagaacagctgcagaacaggagat(/c)aacagttgaatgaaatgttaaaggattcaacacaatggctggaagctaaggaagaagccgaacaggtcataggacaggtcagaggcaagcttgactcatggaaagaaggtcctcacacagtagatgcaatccaaaagaagatcacagaaaccaag NGG 10 8 17 8 24 0 100 5 10
    240114130736_pisj CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240114052212_x0pi TATTTCTGCTGCAAGTAAGCATGCATTTGTAGGCTTGATGCTTTTTTTCTGCTTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGAGGAAGCTAGCCTCTG(+A)TGATGGCAGAGGAAAGGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAGCTTTTCCTAGACAGGGGCTAGTATGTGCAGCTCCTGCACCGGGATACT NGG 10 8 17 8 24 0 100 5 10
    240112064242_zo6i CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240111114247_8tjc ACAAGCTTAAGTGTCTCGATTACCACACCCCTCCCAACCCCTCAGGCCTCAATGGAC(-AA)AAGAGCTCTTGCTGCATCGTAGCCACCAGTACTCAGGGCAGTGTGCTCCCGGCCTGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240111053831_24ry GTAAAGTAGGAGAAAGTCTAAATCTTAATTTGAGTTTATGTCAAGAACAGTGTTTGTGGTATTGGTTTTGTAGCCAACTTTATACTTCAGCATTAAGTTTAAGTCTGCTAAATTTCTAACTTGTTTCAGAGTTGTTAGATTTTATTTTGAGGTCTATTTCCAATTGCTTTGGTATCCATGACCCTTACAACAAATTACACATTTACTGAATGCTTATTTTTCACTATCCTTAAGTCAGTACATTTCAGGGTAGAAGTTATATCAAGCACATGAAAAGTCTTGAGTAGCTTCATTTATAAATACTCCCATATATAAATATGCTTAAAAGTTTGATTTTTAGAAACAGTTGTGTGTTTTTAAGATAACAAAAAGTATTATTTTTCAATAGGAGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTCAAAAAA(/G)GGTGAGTTTTAGTGAAAGTATTATTTAGCACATCTCTAACATATTTTCCAGACTATATTCAAATAATATTGCTGCAAACACCAGTATAAAGATAGTTTATAAAACAAAGCTGTTCTTCGGTTCTAGGAAATGACCCACAGAAACAATAGTTTTACTTCAGTGACTTCCTTGCCACAGTAACTATAACTTGTTATCCCTCCCGTTACTGTAATAATGATGCTTCTGTGAACTATCCTGCAGTAGAAATGTACTGTTGGTGTTTTAGATTTAAATATGTGTGCACTCATCTTAAATATATCGCCACAGTTATTTTCAGCAAAGGGCACACGGTAGTCAGGGGACCTGACTATCTATTCCTGACTCTAGAAGTCTATTttagtcttctcgtgatgaagataacttacttaccttatcagagttgttgaggattacataagTATGTTGATAATAGGCTTTTTTAAAGTAGCACTAGAATTTTAGTGTTCTAAGAGAGATTTACAGCCATATTCTCTA NG 50 8 17 8 24 0 100 5 10
    240111053322_mmcu GTAAAGTAGGAGAAAGTCTAAATCTTAATTTGAGTTTATGTCAAGAACAGTGTTTGTGGTATTGGTTTTGTAGCCAACTTTATACTTCAGCATTAAGTTTAAGTCTGCTAAATTTCTAACTTGTTTCAGAGTTGTTAGATTTTATTTTGAGGTCTATTTCCAATTGCTTTGGTATCCATGACCCTTACAACAAATTACACATTTACTGAATGCTTATTTTTCACTATCCTTAAGTCAGTACATTTCAGGGTAGAAGTTATATCAAGCACATGAAAAGTCTTGAGTAGCTTCATTTATAAATACTCCCATATATAAATATGCTTAAAAGTTTGATTTTTAGAAACAGTTGTGTGTTTTTAAGATAACAAAAAGTATTATTTTTCAATAGGAGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTCAAAAA(/G)AGGTGAGTTTTAGTGAAAGTATTATTTAGCACATCTCTAACATATTTTCCAGACTATATTCAAATAATATTGCTGCAAACACCAGTATAAAGATAGTTTATAAAACAAAGCTGTTCTTCGGTTCTAGGAAATGACCCACAGAAACAATAGTTTTACTTCAGTGACTTCCTTGCCACAGTAACTATAACTTGTTATCCCTCCCGTTACTGTAATAATGATGCTTCTGTGAACTATCCTGCAGTAGAAATGTACTGTTGGTGTTTTAGATTTAAATATGTGTGCACTCATCTTAAATATATCGCCACAGTTATTTTCAGCAAAGGGCACACGGTAGTCAGGGGACCTGACTATCTATTCCTGACTCTAGAAGTCTATTttagtcttctcgtgatgaagataacttacttaccttatcagagttgttgaggattacataagTATGTTGATAATAGGCTTTTTTAAAGTAGCACTAGAATTTTAGTGTTCTAAGAGAGATTTACAGCCATATTCTCTA NG 50 8 17 8 24 0 100 5 10