Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240111053230_creu GTAAAGTAGGAGAAAGTCTAAATCTTAATTTGAGTTTATGTCAAGAACAGTGTTTGTGGTATTGGTTTTGTAGCCAACTTTATACTTCAGCATTAAGTTTAAGTCTGCTAAATTTCTAACTTGTTTCAGAGTTGTTAGATTTTATTTTGAGGTCTATTTCCAATTGCTTTGGTATCCATGACCCTTACAACAAATTACACATTTACTGAATGCTTATTTTTCACTATCCTTAAGTCAGTACATTTCAGGGTAGAAGTTATATCAAGCACATGAAAAGTCTTGAGTAGCTTCATTTATAAATACTCCCATATATAAATATGCTTAAAAGTTTGATTTTTAGAAACAGTTGTGTGTTTTTAAGATAACAAAAAGTATTATTTTTCAATAGGAGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTCAA(/G)AAAAGGTGAGTTTTAGTGAAAGTATTATTTAGCACATCTCTAACATATTTTCCAGACTATATTCAAATAATATTGCTGCAAACACCAGTATAAAGATAGTTTATAAAACAAAGCTGTTCTTCGGTTCTAGGAAATGACCCACAGAAACAATAGTTTTACTTCAGTGACTTCCTTGCCACAGTAACTATAACTTGTTATCCCTCCCGTTACTGTAATAATGATGCTTCTGTGAACTATCCTGCAGTAGAAATGTACTGTTGGTGTTTTAGATTTAAATATGTGTGCACTCATCTTAAATATATCGCCACAGTTATTTTCAGCAAAGGGCACACGGTAGTCAGGGGACCTGACTATCTATTCCTGACTCTAGAAGTCTATTttagtcttctcgtgatgaagataacttacttaccttatcagagttgttgaggattacataagTATGTTGATAATAGGCTTTTTTAAAGTAGCACTAGAATTTTAGTGTTCTAAGAGAGATTTACAGCCATATTCTCTA NG 50 8 17 8 24 0 100 5 10
    240111052643_zwqf ATATTTGGCTTTTTTATGTTTAAATATTCTCATCAGCTGTATTGAATTTGTCTAGGTTTAACTAATTGTAACTTAAACTCTAGGTTACAAGAGCATTCTCTTTCTCCAAAACTCCAAAAA(/G)GAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTAGCAGTAAGTTATTTTGATTTAATGGG NG 50 8 17 8 24 0 100 5 10
    240111052559_yg04 GAGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTCAAAAA(/G)AGGTGAGTTTTAGTGAAAGTATTATTTAGCACATCTCTAACATATTTTCCAGACTATATTCAAATAATATTGCTGCAAACACCAGTATAAAGATAGTTTATAAAACAAAGCTGTTCTTCGGTTCTAGGAAATGACCCACAGAAACAATAGTTTTACTTCAGTGACTTCCTTGCCACAGTAACTATAACTTGTTATCCCTCCCGTTACTGTAATAATGATGCTTCTGTG NG 10 8 17 8 24 0 100 5 10
    240111052537_hja2 GAGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTCAA(/G)AAAAGGTGAGTTTTAGTGAAAGTATTATTTAGCACATCTCTAACATATTTTCCAGACTATATTCAAATAATATTGCTGCAAACACCAGTATAAAGATAGTTTATAAAACAAAGCTGTTCTTCGGTTCTAGGAAATGACCCACAGAAACAATAGTTTTACTTCAGTGACTTCCTTGCCACAGTAACTATAACTTGTTATCCCTCCCGTTACTGTAATAATGATGCTTCTGTG NG 10 8 17 8 24 0 100 5 10
    240111052420_3mej ATATTTGGCTTTTTTATGTTTAAATATTCTCATCAGCTGTATTGAATTTGTCTAGGTTTAACTAATTGTAACTTAAACTCTAGGTTACAAGAGCATTCTCTTTCTCCAAAACTCCAA(/G)AAAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTAGCAGTAAGTTATTTTGATTTAATGGG NG 10 8 17 8 24 0 100 5 10
    240111052344_smbt AAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCT(/C)ACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGG NGG 10 8 17 8 24 0 100 5 10
    240111052133_8nmq ATATTTGGCTTTTTTATGTTTAAATATTCTCATCAGCTGTATTGAATTTGTCTAGGTTTAACTAATTGTAACTTAAACTCTAGGTTACAAGAGCATTCTCTTTCTCCAAAACTCCAA(/G)AAAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTAGCAGTAAGTTATTTTGATTTAATGGG NGG 10 8 17 8 24 0 100 5 10
    240111052000_ff83 AAGAGATTCTAATCCTGCCACATAGTACAGTAGAAAAACTTTTGCCATATATTTGGCTTTTTTATGTTTAAATATTCTCATCAGCTGTATTGAATTTGTCTAGGTTTAACTAATTGTAACTTAAACTCTAGG(/C)TTACAAGAGCATTCTCTTTCTCCAAAACTCCAAAAAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTAGCAGTAAGTTATTTTGATTTAATGGGGTAAATGACTTATTTATGAAGTTGTTATGGAATTTGTTAACTTCTAAAAATTGGAAATATTT NGG 10 8 17 8 24 0 100 5 10
    240111051640_g5yx gttgaggggatttttggattagaaatctcacaactagactggctgaatcagagaagaacttaggtctcttctctcccaggctagatttcttttctcCTCCTCCTTCCATTGGACTGCAGTGCTCTCTAAATGAAAGTTTAAATAGTCTACAAATTTCCTCACTGTTTTGTGCCAAAGAGATTCTAATCCTGCCACATAGTACAGTAGAAAAACTTTTGCCATATATTTGGCTTTTTTATGTTTAAATATTCTCATCAGCTGTATTGAATTTGTCTAGGTTTAACTAATTGTAACTTAAACTCTAGGTTACAAGAGCATTCTCTTTCTCCA(/G)AAACTCCAAAAAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTAGCAGTAAGTTATTTTGATTTAATGGGGTAAATGACTTATTTATGAAGTTGTTATGGAATTTGTTAACTTCTAAAAATTGGAAATATTTC NGG 10 8 17 8 24 0 100 5 10
    240111051434_vq8p CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 50 8 17 8 24 0 100 5 10