Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240111051232_r28m CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240111050533_f3w7 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240110234001_v67l CAGGCTACACTCTTCTTGAAGGTGTAGGCTTTGGGCAAAAGCATTCATTCAGTAACCCCAGAAACTTGTTCTGTTTTTCCATAGGATTCTCTTTGGACAGT(/C)GTCCATACTGGTGGGTTTTGGATACTGACTACTACAGCAACACTTCCGTGCCCCTGATAAAGCAGTTCCCTGTAACCTGTGAGACTGGACCAGGTAAGCGTC NGG 10 8 17 8 24 0 100 5 10
    240110233551_rqz3 ACCCCCATCCCAAGTCGAGCTGGTCTTCTACGTCTTGTCCTTCTGCAAGAGTGCGGTAGTGCCCCTGGCATCCGTCAGTGTCATCCCCTACTGCCTCGCCT(/C)AGGTCCTGGGCCAGCCGCACAAGAAGTCGTTGTAAGAGATGTGGAGTCTTCGGTGTTTAAAGTCAACAACCATGCCAGGGATTGAGGAGGACTACTATTTGA NGG 10 8 17 8 40 0 100 5 10
    240110233519_nq3u ACCCCCATCCCAAGTCGAGCTGGTCTTCTACGTCTTGTCCTTCTGCAAGAGTGCGGTAGTGCCCCTGGCATCCGTCAGTGTCATCCCCTACTGCCTCGCCT(/C)AGGTCCTGGGCCAGCCGCACAAGAAGTCGTTGTAAGAGATGTGGAGTCTTCGGTGTTTAAAGTCAACAACCATGCCAGGGATTGAGGAGGACTACTATTTGA NGG 10 8 17 8 24 0 100 5 10
    240110075852_nb84 GATGCTTCTCACCAGCCTCATTTTGTGCTCCTTGGTCCTGGGAGTCAGCAGTTGGTTTTCATTCATTGGTGAGGCTTACCCAAAGGAAGACAGCTGGAAAAAT(-TCAGACAAATACTTCCATGCTCGGGGAACTATGATGCTGCCCAAA)GGGGTATCAGGGGAGCCTGGGCGGCTGAAGTCATCAGTGATGCAAAAGAGGGCATTCAGGCATTCTTCAGCAGTGGACACAAGGACTCCATGGCTGATCAGGAAGCCAACAGACATGACTGCAGTGGCAAGGACCCCAATCACTACAGACCTGCTGGCCTGCCTGACAAATA NGG 10 8 17 8 24 0 100 5 10
    240110063809_p55b CAGGCTACACTCTTCTTGAAGGTGTAGGCTTTGGGCAAAAGCATTCATTCAGTAACCCCAGAAACTTGTTCTGTTTTTCCATAGGATTCTCTTTGGACAGT(/C)GTCCATACTGGTGGGTTTTGGATACTGACTACTACAGCAACACTTCCGTGCCCCTGATAAAGCAGTTCCCTGTAACCTGTGAGACTGGACCAGGTAAGCGTC NGG 10 8 17 8 24 0 100 5 10
    240110063235_33ku TACTTACTGTTAATGGAATCAGCCAAAATCTTAAGCTGCTGGGTATTGTCCAAGACCTCAATCCTTTGGGTGTATGGGTCGTAGCGAACTGAGAAGGGCCA(/G)AGGTATTGTGGCAGCAAAGTTCCTAAGACCAAAACCACAGGCTTGAGTGAAGGGCACCATTTGGAGAAAGGTAGTCTTAAGAGAGTTCTCAGTGGCATTT NGG 10 8 17 8 24 0 100 5 10
    240110062339_odgr AGGACATGGTGAGGAATAAAAGAGCATTGGCGGGGAGGGCAGGGCCACGCCCAAGTCCACGTACCTCTTTACAGTATTTGGTGACTGCCACGCCCAAGGGT(/G)TGTTCACTGCTGGCCTCCGCAGTCCCCACCACAGCCAGAACCTTCCTGAGGGGCAGTGTGGCCACATCCCCCAGCAGGAGCACCCGCATGACCCTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240110062221_u5it AGGACATGGTGAGGAATAAAAGAGCATTGGCGGGGAGGGCAGGGCCACGCCCAAGTCCACGTACCTCTTTACAGTATTTGGTGACTGCCACGCCCAAGGGG(/T)TGTTCACTGCTGGCCTCCGCAGTCCCCACCACAGCCAGAACCTTCCTGAGGGGCAGTGTGGCCACATCCCCCAGCAGGAGCACCCGCATGACCCTGGGGA NGG 10 8 17 8 24 0 100 5 10