Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240120045913_yqgi CGCGGTCGGTCTGGAGAAGGATGTCCTGGACAGCGCAGTCCCGATG(+GACTACAAAGACGATGACGACAAGTAG)TGCGGCCGCATGATCCACGACCCTAAAGGGAACACATCTCCTATCATTTACGACCCTGTCTACAAT NGG 10 8 17 8 45 0 100 5 10
    240120041439_zxsc TGCGCTCTCCGCCCGTGGACGAACGGCCCTGCGCGCGGTCGGTCTGGAGAAGGATGTCCTGGACAGCGCAGTCCCGATGTG(+C)CGGCCGCATGATCCACGACCCTAAAGGGAACACATCTCC NGG 10 8 17 8 24 0 100 5 10
    240119234302_towg GGTTGAACCCAAGGAATCTGAGACATGGTCCCTGAGTATACCAGCCTGGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTACTTC(/T)GAGGTTCTCGGGGCCCATCCACTGTGATTTTGATGGCTCTGTGGTAGGTGGCGACTTGCGGTGGGTTTGTGAAGACAGTGATGGTCAGAGTGAAGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240119123106_1919 AAAGGGCAGCGCCGAGAGGAACGAGCCGGGAGACGCCGGACGGCCGAGCGGCAGGGCGCTCGCGCGCGCCCACTA(/G)GTGGCCGGAGGAGAAGGCTCCCGCGGAGGCCGCGCTGCCCGCCCCCTCCCCTGGGGAGGCTCGCGTTCCCGCTGCTCGCGCCTGCGCCGCCCGCCGGCCTCAGGAACGCGCCCTCTTCGCCGGCGCGCGCCCTCGCAGTCACCGCCACCCACCA NGG 10 8 17 8 24 0 100 5 10
    240119123103_xg2l AAAGGGCAGCGCCGAGAGGAACGAGCCGGGAGACGCCGGACGGCCGAGCGGCAGGGCGCTCGCGCGCGCCCACTA(/G)GTGGCCGGAGGAGAAGGCTCCCGCGGAGGCCGCGCTGCCCGCCCCCTCCCCTGGGGAGGCTCGCGTTCCCGCTGCTCGCGCCTGCGCCGCCCGCCGGCCTCAGGAACGCGCCCTCTTCGCCGGCGCGCGCCCTCGCAGTCACCGCCACCCACCA NGG 10 8 17 8 24 0 100 5 10
    240119122953_slfk AGCGGCAGGGCGCTCGCGCGCGCCCACTA(/G)GTGGCC NGG 10 8 17 8 24 0 100 5 10
    240119120612_l6in GAGGATAGGCTGTGCCTCCTCCCACTCCCGCCCCCAG(/T)CCCCCCTTTTTTTCCCCCTCGGAACGCGAGG NGG 10 8 17 8 24 0 100 5 10
    240119120001_pvue GAGGATAGGCTGTGCCTCCTCCCACTCCCGCCCCCAG(/T)CCCCCCTTTTTTTCCCCCTCGGAACGCGAGG NGG 10 8 17 8 24 0 100 5 10
    240119115549_nzl5 AGCCGGGAGACGCCGGACGGCCGAGCGGCAGGGCGCTCGCGCGCGCCCACTA(/G)GTGGCCGGAGGAGAAGGCTCCCGCGGAGG NGG 10 8 17 8 24 0 100 5 10
    240119113206_87wx GGAAAGAGACCTTAGCCAGGGGGGCGGTTTCGGGACTACGAAGGGTCGGGGCGGACGGACTCGAGGGCCGG(/C)CCACGTGGAAGGCCGCTCAGGACTTCTGTAGGAGAGGACACCGCCCCAGGCTGACTGAAAGTAAAGGGCAGCGGACCCAGCGGCGGAGCCACTGGCCTTGCCCCGACCCCGCATGGCCCGAAGGAGGACACCCACCCCCGCAACGACACAAAGACTCCAACTACAGGAGGTGGAGAAAGCGCGTGCGCCACGGAACGCGCGTGCGCGCTGCGGTCAGCGCCGCGGCCTGAGGCGTAGCGGGAGGGGGACCGCGAAAGGGCAGCGCCGAGAGGAACGAGCCGGGAGACGCCGGACGGCCGAGCGGCAGGGCGCTCGCGCGCGCCCACTAGTGGCCGGAGGAGAAGGCTCCCGCGGAGGCCGCGCTGCCCGCCCCCTCCCCTGGGGAGGCTCGCGTTCCCGCTGCTCGCGCCTGCGCCGCCCGCCGGCCTCAGGAACGCGCCCTCTTCGCCGGCGCGCGCCCTCGCAGTCACCGCCACCCACCAGCTCCGGCACCAACAGCAGCGCCGCTGCCACCGCCCACCTTCTGCCGCCGCCACCACAGCCACCTTCTCCTCCTCCGCTGTCCTCTCCCGTCCTCGCCTCTGTCGACTATCAGGT NGG 10 8 17 8 24 0 100 5 10