Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240123023617_zri3 ATGCAAGATAATCCAACAAAGCATTAATGAATCATAATCATACCTACCGGCTTAGTAAAAATTATGAAGAACTATTTATTCGATTTTCTAAATTCTTTCAGTTGATAACCCAGTCTGAGATATAGCAACACTGACGTTCTCCTGTGGTGTGCAACGTTATACTAATGTTCAAGTTCGGGTTCAAGGCCAGCCTT(+GACTACAAAGACGATGACGACAAG)AACTTCTACAATGGAAGCAGGAAATCGAAGAAGGAACGAGATGATATCGGAGCGATTTCATCGGTCACCGGGGCCTACAGGACGAATAGAGATGAATTCTTTGGTAAATATATTTTAAATATGGATTATTTTTAAAAGTGGTA NGG 10 8 17 8 50 0 100 5 10
    240122203735_dk7n CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATTGGAGACGATGATGTCCCGTAATAGCGAT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 60 0 100 5 10
    240122203630_uh8r CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATTgagatgagc)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 55 0 100 5 10
    240122203550_n9r4 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATTgactgta)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240122200505_6b7t CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATTGGCGTGGT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240122183850_da3q CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240121163655_m676 GCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAAGATGC(/G)TGTGGCCGATGTGTCTATTGAAGATTCTGTGATCTCACTCTCAGGAGACCATTGCATCATTGGCCGCACACTGGTG NGG 10 8 17 8 24 0 100 5 10
    240121044912_rwmx CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240121044209_bckp agcctcctagaggaatcctgctttacggacctcctggaacaggaaagaccctgattgctcgagctgtagcaaatgaga(/g)ctggagccttcttcttcttgatcaatggtgagatatttggttcatcttatgtctagctagacccaattttgaactgggcttatgagctggagcacttatgaa NGG 10 8 17 8 24 0 100 5 10
    240120234507_u6p5 AACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCTCCAAGGAG(+atatggggaagtgaatcagtacaaccgccacagtac)TAAgaaaccctggaccacccaccccagcaaggacactgagcaagagaggccctatcccaactcggcccccaacactgagcatctccctcacaatttccatcccagacccccataataacaggaggggcctagggagccctccctactctcttgaataccatcaataaagttcgctgcaccc NGG 90 8 17 8 50 0 100 5 10