Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231229160350_83dd ccccaccagccctctttccagaaatttccttcttcatccaagggacttttcctcccagaacccgacacagacaccatcaactgcgaccagttcagcaggctgttgtgtgacatggaaggtgatgaagagaccaggg(+GTTAG)aggcttatgccaatatcggtgaggaagcacctgagcccagaaaaggacaatcaagggcaagagttctttgctgccacttgtcaatatcacccattcatcatgagccacgtcagtcccctcccacagaaatcattgcaagggggatgcggagcaatggctggaggaacggagactccagggaagagaggggagat NGG 10 8 17 8 24 0 100 5 10
    231229125933_dfe0 CAGCCTCATGGTGGTGGCTGGGGTCAAGGAGGTGGCACCCACAGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGG(/T)CTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCGGCAGTGACTATGAGGACCGTTACTATCGTGAAAACATGCACCGTTACCCCAACCAAGTGTACTACAGGCCCATGGATGAGTACAGCAACCAGAACAACTTTGTGCACGACTGCGTCAATATCACAATCAAGCAGCACACGGTCACCACAACCACCAAGGGGGAGAACTTCACCGAGACCGACGTTAAG NGG 10 8 17 8 24 0 100 5 10
    231229125552_t1ts GATTGACATGCATTTCGACCAATAGCATTGCAGAGAGGCGTATCATTTCGCGGATGTTCCAATCAGTACGCAGAGAGTCGCCGTCTCCAAGGTGAAAGCGGAAGTAGGGCCTTCGCGCACCTCATGGAATCCCTTCTGCAGCa(/t)CCTGGATCGCTTTTCCGAGCTTCTGGCGGTCTCAAGCACTACCTACGTCAGCACCTGGGACCCCGCCACCGTGCGCCGGGCCTTGCAGTGGGCGCGCTACCTGCGCCACATCCA NGG 10 8 17 8 24 0 100 5 10
    231229125046_uv5m AAACATGGATGTTTATCAAAGAAAGGATTGTCATCCAAAGCACCAACTATGAGAGATATCTATGTGCAATGGTATATAGATCTGTCATAGAAGGGTTTAATTATATCTGCCTAATGATTTTCTCTCCTTAATGCCTCTATAGCTGTCc(/a)CTTAGCGAAGAATAAAACTGTGGACTGACCCCCACCCATTTGCGAAGAAAGTACTGGGTCTTCAGCTTTCATTGTTCAGCCGGTGGTCTTTGTGGACAACAC NGG 10 8 17 8 24 0 100 5 10
    231229124907_0du7 TAATAGCTACTATTGATTAAGTTCTTATGTTGGGTTAAGTACCATGCTAAGCACTTTCCAAAGATTATCTAATTCTTATGTCATCTATATTTTTGTTGGTGCTATTACTCTt(/c)CTCACTTTACTAAGGAAGAAACCAAGACATGGGGTTAAATAACTTCCCTATAAATTTTGAATTATCTTTGGCATCATCTCCCTATTTGCAAATCTCCATTGTCTCTTTGTTCGTAATC NG 10 8 17 8 24 0 100 5 10
    231229124731_3ha3 GTAGCCTGGTATAGTGCTCAGTCAGCCAGGGTGGTTATCTCCAATTATACCCAGCAGGATTAAGAACCTAATGCCCATTAAGTTCTCCATGGCTGGTGAGTGAGTGCCTGTGAGAGAGATAGAAGAGAATGTGATAATGG(/a)GACTATCTCATGTGCTGAGATGTGAGTAGACTATGAGCCCCAGGCAGTCTACTATGTAAATGGTGGGAAGGATGGTATTGTCTGTCACTATATTATCAAGATCTACCATAACAATG NGG 10 8 17 8 24 0 100 5 10
    231229124446_11fz CTGTGTCCCGGCTTTGTGCTTCTCCCGAATCAGCTTCGTTTGGTTCCTTGGTAAGTGACAGGCAGACACAAAGGCAGGCGCAGGCCCGGGGAGGGGGCGGGAGGGGG(/t)TGGGGAGCGCAGCGTTGGAGTTGCAAGACTGCAAGGTCAGGGGCGCCTAAAGAAATGAAACCCAATCCCAGCAAAGAAGTGAAGAGCAGATTTATAACAGTCCCATCCAAATTTCTCTTTGGCTTCTCTC NGG 10 8 17 8 24 0 100 5 10
    231229123328_kf85 TATCTGGAGCCCATTTGGGGCTTAGGAGGAAGAGGGAGAAGGGTCAGCTTTGGAATACCACGGACTTGCTGGGAGACCCCCTAGCAAGGAGCGGTGCAAACAACCTTGTCCTCTCg(/a)GAACCTCAGTTTTCCACTCTGTCAAATGGGTGCTTGTGACACATTGAGAAGCAGATGGGTGTAACATACTCTCAGATGTGAGGACTGCCCAAGTTATCAAGTGCCAGGCT NGG 10 8 17 8 24 0 100 5 10
    231229122045_35gn GCTAGGAACTCGTCCACCGTCAGCTCCGCCAGGCGCCTGCGGGTCACGCAGGAGTCACAGCTGCCCGCACGCCCAGCTCGCCCCAGCCCCGCTGAGAGGAGCAAGAAAAGCCCCCTTGGATACAGACACCCACCGGGAGGCCAAATCa(/g)GCCCTCGGACCCGCGGCTTACCTCTTGCGGCTCCCCGCAGCTGCCATGACACCAACCCGAAGCGTGCACCCCACTTCCGGCCCCAGAATGCCGCGCGGCTGCGCACTTCCGC NGG 10 8 17 8 24 0 100 5 10
    231229121518_lwwj CCGGCTTTGTGCTTCTCCCGAATCAGCTTCGTTTGGTTCCTTGGTAAGTGACAGGCAGACACAAAGGCAGGCGCAGGCCCGGGGAGGGGGCGGGAGGGGG(/T)TGGGGAGCGCAGCGTTGGAGTTGCAAGACTGCAAGGTCAGGGGCGCCTAAAGAAATGAAACCCAATCCCAGCAAAGAAGTGAAGAGCAGATTTATAACAGTCCCATCCAAATTTCTCTTTGGCTTCTCTCT NGG 10 8 17 8 24 0 100 5 10