Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231226093721_ugs5 TAATTCATAGCAGTGTTTCTATTAATATCCTTTGGATTCTGAATGAGATGCAAACTAGTTTCAATCTCCTTAAAAATCATGTGAAAACATACCTGGAATTTTTCACCTTTGTGCATCTGAGTTGATCTAAATTCAGGAG(/A)AATCTATAAAGGCACAGGGGTAAATGTTATGCAGAAAATGTCCTCGATAGG NGG 10 8 17 8 24 0 100 5 10
    231226093348_qjrr AAACAGGGCTCTGCTGGGCCCCAAGGTCCTCCTGGTCCCAGTGGTGAAGAAGG(/A)AAAGAGAGGCCCTAATGGGGAAGCTGGATCTGCCGGCCCTCCAGGACCTCCTGGGCTGAGAGTAGGTTTCAAATGCTCCCAACACCCTAACACACCAGAGGCAGATTATGATACCCCTTCACTGGGAATTGGTCAAAATTACTGACT NGG 10 8 17 8 24 0 100 5 10
    231226093038_d7yt CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231226071053_x0f9 GGCTCGTACAAAGCAGACTGCCCGCAAATCGACCGGTGGTAAAGCACCCAGGAAGCAACTGGCTACAAAAGCCGCTCGCAAGAGTGCGCCCTCTACTGGAG(/A)GGGTGAAGAAACCTCATCGTTACAGGTATTAAAAAACAGGAAAAAAATGGGACAAAGTCTCTCTTGTATGTATCCACATAATTTAACAAAAAGATGGATA NGG 10 8 17 8 24 0 100 5 10
    231225081824_2v09 CTGAGAAGGCGGAGAGGAGCCCTGTGACATTCTTCGACACGCCCCCCATGCTCTTTGTGTTCATTGCCCTGGGCCT(/G)GTGGCTGGAACACTTGGCAAAGGTAACAGCAGCTTCAGGTTCAGAAAAGAGCTGCTCCTTCAGTAAACAAATCTC NGG 10 8 17 8 24 0 100 5 10
    231224232600_l6qs TCAGCCAACATGGACGTGCTCATCGTCCTGGCCACAAGCATTGCTTATGTTTATTCTCTGGTCATCCTGGTGGTTGCTGTGGCTGAGAAGGCGGAGAGGAGCCCTGTGACATTCTTCGACACGCCCCCCATGCTCTTTGTGTTCATTGCCCTGGGCCT(/G)GTGGCTGGAACACTTGGCAAAGGTAACAGCAGCTTCAGGTTCAGAAAAGAGCTGCTCCTTCAGTAAACAAATCTCACTTCCT NGG 10 8 17 8 24 0 100 5 10
    231224232534_6okv TCAGCCAACATGGACGTGCTCATCGTCCTGGCCACAAGCATTGCTTATGTTTATTCTCTGGTCATCCTGGTGGTTGCTGTGGCTGAGAAGGCGGAGAGGAGCCCTGTGACATTCTTCGACACGCCCCCCATGCTCTTTGTGTTCATTGCCCTGGGCCG(/T)GTGGCTGGAACACTTGGCAAAGGTAACAGCAGCTTCAGGTTCAGAAAAGAGCTGCTCCTTCAGTAAACAAATCTCACTTCCT NGG 10 8 17 8 24 0 100 5 10
    231222161718_13w9 GACATCCTGGCTACTAGCTTCCTCTACGGCAGCAAGACCTTCATCAACCACACCC(/T)AGGGCATCCCCGACTTCTTCAAGCAGTCCTTCCCTGAGGGCTTCACATGGGAGAGAGTCACCACATACGAA NGG 10 8 17 8 24 0 100 5 10
    231221183851_1maj CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231221164204_q9j5 atggacctcttcccctattaccggcagaaccagcagcgctggcagaactccatccgccacT(/G)CGctgtccttcaatgactgcttcgtcaaggtggcacgctccccggacaagccgggcaagg NGG 10 8 17 8 24 0 100 5 10