Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231206204751_6k5z cacaggaaaatctgaacaagctgatgaccaacttgc(/T)gctccacacatccccacttcgtgcgctgcatcatccccaatgagacaaagtctcc NGG 10 8 17 8 24 0 100 5 10
    231206161048_8xx7 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231206161039_1xcj CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231206103309_4u6k gcccaggaccagccggtgaccctaggggtcctggggaccaactttggccgctgcgtggacctctttgccccaggtgatgacatcattggtgcctccagcG(/T)actgcagcacttgcttcacgtcgcagagcgggacgtcacaggctgccgcccatgtggctggtgagttgctgccccacccctcaaccaacatgattctgac NGG 10 8 15 8 24 0 100 5 10
    231206102322_hxsc tgcccaggaccagccggtgaccctaggggtcctggggaccaactttggccgctgcgtggacctctttgccccaggtgatgacatcattggtgcctccagcg(/t)actgcagcacttgcttcacgtcacagagtgggacgtcacaggctgccgcccatgtggctggaattgtgaccatgatgctgacggctgagccggagctcac NGG 50 8 15 10 20 50 90 5 10
    231206100715_mujh cactggtcgagccagtgcagatgatctgtgatgacgcgaagtatgtgaatgtgacggcagtggacctggacggaccccagaacagcgcacctttcagttt(/g)ctctatcattgacaaaccagcaggaatggcagaaagatggaaaatagtacaccaagaaagtaccagtgtactattacagcaaaacgaacaaaaggctgg NG 50 8 15 10 20 50 90 5 10
    231206100330_hhmi cactggtcgagccagtgcagatgatctgtgatgacgcgaagtatgtgaatgtgacggcagtggacctggacggaccccagaacagcgcacctttcagttt(/g)ctctatcattgacaaaccagcaggaatggcagaaagatggaaaatagtacaccaagaaagtaccagtgtactattacagcaaaacgaacaaaaggctgg NG 10 8 17 8 24 0 100 5 10
    231206093545_zbd0 agtatgtgaatgtgacggcagtggacctggacggaccccaga(/t)acagcgcacctttcagtttctctatcattgacaaaccagcaggaatggcagaaagatggaaaatagtacaccaagaaag NGG 10 8 17 8 24 0 100 5 10
    231206093335_b8o2 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231205225832_dv8u CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10