Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231205163410_qid4 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    231205163405_wm0n CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    231205163324_t1q0 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231205154400_wnna AACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACAC NG 10 8 17 8 24 0 100 5 10
    231205153630_d8uj AACTGTGAAGACAGTTACAGCAGTTAT(+C)GGTGTGCCTGTCTGTCGGGATGGTCAGGGACACACT NGG 10 8 17 8 24 0 100 5 10
    231205151103_l4ft ttctttctctcctctcccttttttaaacacctctttctttcactttcccctcttgtgttctgggccacaggaaaatctgaacaagctgatgaccaacttgc(/t)gctccacacatccccacttcgtgcgctgcatcatccccaatgagacaaagtctccaggtgagccaagtagtcgggccaagcctgctgctggaaacccagg NGG 10 8 17 8 24 0 100 5 10
    231205124844_d1io AACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACAC NGG 10 8 17 8 24 0 100 5 10
    231205123745_76jv AGTCTCCTCT(/C)GATGTGGGCCAGGGCTCTTTGAAGTTGGATCTG NGG 10 8 17 8 24 0 100 5 10
    231205102159_m8fr acctgttgagaagggcaaaggcaaggccaagaaaggctcatcctttcagactgtgtcagctctgcacagggaaaatctgaacaagctgatgaccaacttgc(/t)gctccacacatccccacttcgtgcgctgcatcatccccaatgagacaaagtctccaggggtaatagacaatcctctggtcatgcaccagctgcgctgcaa NGG 10 8 17 8 24 0 100 5 10
    231205101833_6ibr acctgttgagaagggcaaaggcaaggccaagaaaggctcatcctttcagactgtgtcagctctgcacagggaaaatctgaacaagctgatgaccaacttgc(/t)gctccacacatccccacttcgtgcgctgcatcatccccaatgagacaaagtctccaggggtaatagacaatcctctggtcatgcaccagctgcgctgcaa NGG 10 8 17 8 24 0 100 5 10