Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231209164011_56y8 acagagggcacatggaggatatttgagagcctgtagaccaggaaggtagcctaacctctgaccccatccccccaggagaaccgggaggtgggagactggcG(/A)caagaacatcgacgcgctcagtgggatggaaggtcgcaagaaaaagtttgagggctgagctggcctgacgggcagcctggccccaaccctgaggaataa NGG 10 8 17 8 24 0 100 5 10
    231209150114_kgdd CAATAGAGATTGGAGGCATATATCTTTCTTACTTTGAAAATCTACATGGTTTCCCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACC NG 10 8 17 8 24 0 100 5 10
    231209145821_rx0z TGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    231209145150_3qwu CAATAGAGATTGGAGGCATATATCTTTCTTACTTTGAAAATCTACATGGTTTCCCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGCTACAGTTCTTAT(+C)GGTGTGCCTGTCTCTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACC NGG 10 8 17 8 24 0 100 5 10
    231209143940_ajt8 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    231209120833_508x gacttttaagagcaactgggggtttggtgccctctggtggctagctcagaaaagaaacccaaactaacagttgtcccaatttgttctaggtatgacaatgaatacggctacagcaacagggtggtggacctcatggcctacatggcCTCCAAGGAGTAAGAAACCCTGGaccacccaccccagcaaggacactgagcaagagaggccctatcccaactcggCCCCCA(+aacc)ACACTGAGCATCTCCCTcacaatttccatcccagacccccataataacaggaggggcctagggagccctccctactctcttgaataccatcaataaagttcgctgcacccacctctccttggttttaatgcggtgttgggggagggagggtgtgtatggggaggtgaagcaggctcaatcaacttttgtgcatgtatctttattggctctgacatccagctgggtgccggaagtccagggctacattctatccaag NGG 10 8 17 8 24 0 100 5 10
    231209115559_iu75 aaacctgacttttaagagcaactgggggtttggtgccctctggtggctagctcagaaaagaaacccaaactaacagttgtcccaatttgttctaggtatgacaatgaatacggctacagcaacagggtggtggacctcatggcctacatggcCTCCAAGGAGTAAGAAA(+AACGTAG)CCCTGGaccacccaccccagcaaggacactgagcaagagaggccctatcccaactcggcccccaacactgagcatctccctcacaatttccatcccagacccccataataacaggaggggcctagggagccctccctactctcttgaataccatcaataa NGG 10 8 17 8 24 0 100 5 10
    231208041952_4ieb CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231207175937_fivn ttaccgttggggccaccaatgcccaggaccagccggtgaccctaggggtcctggggaccaactttggccgctgcgtggacctctttgccccaggtgatgacatcattggtgcctccagcG(/T)actgcagcacttgcttcacgtcacagagtgggacgtcacaggctgccgcccatgtggctggtgagttgctgccccacccctcaaccaacaagattctgacaacccctatggcagccagggtct NGG 10 8 17 8 24 0 100 5 10
    231207120954_1ouh GCCTGGAGGTGTCTGGGTCCCTCCCCCACCCGACTACTTCACTCTCTGTCCTCTCTGCCCAGGAGCCCAGGATGTGCGAGTTCAAGTGGCTACGGCCGAG(/C)GTGCGAGGCCAGCTCGGGGGCACCGTGGAGCTGCCGTGCCACCTGCTGCCACCTGTTCCTGGACTGTACATCTCCCTGGTGACCTGGCAGCGCCCAGATGCACCTGCGAACCACCAGAATGTGGCCGC NGG 10 8 17 8 24 0 100 5 10