Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231204104314_d4tc AACTGTGAAGACAGTTACAGCAGTTAT(+C)GGTGTGCCTGTCTGTCGGGATGGTCAGGGACACACT NGG 10 8 17 8 24 0 100 5 10
    231204092515_phwe GTATCGCAAACGCTGTTTTCAGCGGATTAAGCAGAGAAATACTCTTCAGAAGCAATGGGAACATTACCAGAGAACTCACCAATATCACATTTGCTTTCAGAACACATGATACAAATGTGATGATATTGCATGCAGAAAAAGAACCAGAGTTTCTTAATATTAGCATTCAAGATGCCAGATTATTCTTTCAATTGCGAAGTGGCAACAGCTTTTATACGCTGCACCTGATGGGTTCCCAATTGGTGAATGATGGCACATGGCACCAAGTGACTTTCTCCATGATAGACCCAGTGGCCCAGACCTCCCGGTGGCAAATGGAGGTGAACGACCAGACACCCTTTGTGATAAGTGAAGTTGCTACTGGAAGCCTGAACTTTTTGAAGGACAATACAGACATCTATGTGGGTGACCAATCTGTTGACAATCCGAAAGGCCTGCAGGGCTGTCTGAGCACAATAGAGATTGGAGGCATATATCTTTCTTACTTTGAAAATCTACATGGTTTCCCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTACAAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCATGGAGGAAACTGTGAAGACAGTTACAGCAGTTAT(+C)GGTGTGCCTGTCTGTCGGGATGGTCAGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAACTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAACTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGTTCCTGAAGCTCATGGCTACTCTTGTCTCTGCTTTGGAAATTTTACCGGGAGATTTTGCAG NGG 10 8 17 8 24 0 100 5 10
    231201215939_srad ttaccgttggggccaccaatgcccaggaccagccggtgaccctaggggtcctggggaccaactttggccgctgcgtggacctctttgccccaggtgatgacatcattggtgcctccagcg(/T)actgcagcacttgcttcacgtcacagagtgggacgtcacaggctgccgcccatgtggctggtgagttgctgccccacccctcaaccaacaagattctgacaacccctatggcagc NGG 10 8 17 8 24 0 100 5 10
    231201214558_0m84 ATCATTGCTCCTCCTGAGCGCAAGTACTCCGTGTGGATCGGCGGCTCCATCCTGGCCTCGCTGTCCACCTTCCAGCAGATGTGGATCAGCAAGCAGGAGTATGACGAGTCCGGCCCCTCCATCGTCCACCGCAAATGCTTCT(+tgg)aGGCGGACTATGACTTAGTTGCGTTACACCCTTTCTTGACAAAACCTAACTTGCGCAGAAAACAAGATGAGATTGGCATGGCTTTATTTGTTTTTTTTGTTTTGTTTTGGTTTTTTTTTTTTTTTTGGCTTGACTCAG NGG 10 8 17 8 24 0 100 5 10
    231201214432_ryj4 cctatctctttccctttctttctctcctctcccttttttaaacacctctttctttcactttcccctcttgtgttctgggccacaggaaaatctgaacaagctgatgaccaacttgc(/T)gctccacacatccccacttcgtgcgctgcatcatccccaatgagacaaagtctccaggtgagccaagtagtcgggccaagcctgctgctggaaacccaggaccctgggcatggggccttctaggtcacctcctgatctcc NGG 10 8 17 8 24 0 100 5 10
    231201214411_jhef tggtacgtgggtgaggggatggaggaaggcgagttttcagaggcccgtgaagatatggctgcccttgagaaggattatgaggaggttggtgtggattctgttgaaggagagggtgaggaagaaggagaggaatactaattatccattcc(+AATT)TTTTggccctgcagcatgtcatgctcccagaatttcagcttcagcttaactgacagacgttaaagctttctggttagattgttttcacttggtgatcatgtcttttccatgtgtacctgtaatatttttccatcatatctcaaagtaaagt NGG 10 8 17 8 24 0 100 5 10
    231201095243_gmtp tcactggcactattgttatcatggtcgaagacgtcaatgacaattgtcccacactggtcgagccagtgcagatgatctgtgatgacgcgaagtatgtgaatgtgacggcagtggacctggacggaccccagaacagcgcacctttcagttT(/g)ctctatcattgacaaaccagcaggaatggcagaaagatggaaaatagtacaccaagaaagtaagcaaaagcacggggctacatatttgaatattaaacatgatagaaaccgaaaagctttataatatcgggttttttttcttttcatgaatgatatttccaacacagtg NG 10 8 17 28 35 0 100 5 10
    231130235236_48ft CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231129231150_pdz3 aagtgttccagaagcagattctgcaatgggcatccagaagagagtggcccagcgggcaaaagggcacagagggcacatggaggatatttgagagcctgtagaccaggaaggtagcctaacctctgaccccatccccccaggagaaccgggaggtgggagactggcg(/a)caagaacatcgacgcgctcagtgggatggaaggtcgcaagaaaaagtttgagggctgagctggcctgacgggcagcctggccccaaccctgaggaa NGG 10 8 17 8 24 0 100 5 10
    231129224831_8o7r ccttttttaaacacctctttctttcactttcccctcttgtgttctgggccacaggaaaatctgaacaagctgatgaccaacttgC(/T)gctccacacatccccacttcgtgcgctgcatcatccccaatgagacaaagtctccaggtgagccaa NGG 10 8 17 8 24 0 100 5 10