Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231126192834_1bdv ATGTCTCTCCATCTCCTCCTCTTCTTCTTCCTCCAGCTCTTCAGCTCGTCTCTCCAGCAGTTCCCCCGAGTCTGCACC(/A)TCCCCAGAAGTCCTCCAGTCCAAACGCTGCTGTCCAGTCTGGCCCGGCGACGGCTCCGTGTGCG NGG 10 8 17 8 24 0 100 5 10
    231126192640_5huq ATGTCTCTCCATCTCCTCCTCTTCTTCTTCCTCCAGCTCTTCAGCTCGTCTCTCCAGCAGTTCCCCCGAGTCTGCACC(/A)TCCCCAGAAGTCCTCCAGTCCAAACGCTGCTGTCCAGTCTGGCCCGGCGACGGCTCCGTGTGCGGCGTCCAGTCAGGTCGAGGGTTCTGTCAGGACGTCCTGGTGTCCGACCTTCCCAACGGGCCGCAGTATCCTCACTCAGGAGTGGACGATCGAGAGCGATGGCCTTTAGTGTTTTAC NGG 10 8 17 8 24 0 100 5 10
    231126192139_h32u GTGAAGCCTCTCACTCTCCTCGACTCTTCATCATCATGTCTCTCCATCTCCTCCTCTTCTTCTTCCTCCAGCTCTTCAGCTCGTCTCTCCAGCAGTTCCCCCGAGTCTGCACC(/A)TCCCCAGAAGTCCTCCAGTCCAAACGCTGCTGTCCAGTCTGGCCCGGCGACGGCTCCGTGTGCGGCGTCCAGTCAGGTCGAGGGTTCTGTCAGGACGTCCTGGTGTCCGACCTTCC NGG 10 8 17 8 24 0 100 5 10
    231126192119_ndxm ATGTCTCTCCATCTCCTCCTCTTCTTCTTCCTCCAGCTCTTCAGCTCGTCTCTCCAGCAGTTCCCCCGAGTCTGCACC(/A)TCCCCAGAAGTCCTCCAGTCCAAACGCTGCTGTCCAGTCTGGCCCGGCGACGGCTCCGTGTGCGGCGTCCAGTCAGGTCGAGGGTTCTGTCAGGACGTCCTGGTGTCCGACCTTCC NGG 10 8 17 8 24 0 100 5 10
    231126191916_4wng ATGTCTCTCCATCTCCTCCTCTTCTTCTTCCTCCAGCTCTTCAGCTCGTCTCTCCAGCAGTTCCCCCGAGTCTGCACC(/A)TCCCCAGAAGTCCTCCAGTCCAAACGCTGCTGTCCAGTCTGGCCCGGCGACGGCTCCGTGTGCG NGG 10 8 17 8 24 0 100 5 10
    231126191826_48b9 TCGAGGGTTCTGTCAGGACGTCCTGGTGTCCGACCTTCCCAACGGGCCGCAGTATCCTCACTCAGG(/T)AGTGGACGATCGAGAGCGATGGCCTTTAGTGTTTTACAACCAAACCTGCCAGTGCGCC NGG 10 8 17 8 24 0 100 5 10
    231126191306_qfs4 TCGAGGGTTCTGTCAGGACGTCCTGGTGTCCGACCTTCCCAACGGGCCGCAGTATCCTCACTCAGG(/T)AGTGGACGATCGAGAGCGATGGCCTTTAGTGTTTTACAACCAAACCTGCCAGTGCGCC NGG 10 8 17 8 24 0 100 5 10
    231126191036_fz0t ATGTCTCTCCATCTCCTCCTCTTCTTCTTCCTCCAGCTCTTCAGCTCGTCTCTCCAGCAGTTCCCCCGAGTCTGCACC(/A)TCCCCAGAAGTCCTCCAGTCCAAACGCTGCTGTCCAGTCTGGCCCGGCGACGGCTCCGTGTGCG NGG 10 8 17 8 24 0 100 5 10
    231126190644_nrzp ATGTCTCTCCATCTCCTCCTCTTCTTCTTCCTCCAGCTCTTCAGCTCGTCTCTCCAGCAGTTCCCCCGAGTCTGCACC(/G)TCCCCAGAAGTCCTCCAGTCCAAACGCTGCTGTCCAGTCTGGCCCGGCGACGGCTCCGTGTGCG NGG 10 8 17 8 24 0 100 5 10
    231124212731_ttxp CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10