Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231127004705_9sjf Ggcttagtatacattgaggcacctgcaggaaaattatgctataattcaagacaacagctgaactgtacctctatagaagctatgagcaaatgtgtatggcagatgtc(+TAGTAC)cagaggcgacgaagcgccattgactttgggaccagggagtgaaataatgctttcagatacatgtacagaaatgtctgcagtcacgcttttaaagacaaatggatactggtcagGTGTGTATGCAATCATATGAATAC NGG 10 8 17 8 24 0 100 5 10
    231127004518_kpwc Ggcttagtatacattgaggcacctgcaggaaaattatgctataattcaagacaacagctgaactgtacctctatagaagctatgagcaaatgtgtatggcagatgtc(+TTT)cagaggcgacgaagcgccattgactttgggaccagggagtgaaataatgctttcagatacatgtacagaaatgtctgcagtcacgcttttaaagacaaatggatactggtcagGTGTGTATGCAATCATATGAATAC NGG 10 8 17 8 24 0 100 5 10
    231127004439_uenl Ggcttagtatacattgaggcacctgcaggaaaattatgctataattcaagacaacagctgaactgtacctctatagaagctatgagcaaatgtgtatggcagatgtc(+TAGTAC)cagaggcgacgaagcgccattgactttgggaccagggagtgaaataatgctttcagatacatgtacagaaatgtctgcagtcacgcttttaaagacaaatggatactggtcagGTGTGTATGCAATCATATGAATAC NGG 10 8 17 8 24 0 100 5 10
    231127004258_tkwh Ggcttagtatacattgaggcacctgcaggaaaattatgctataattcaagacaacagctgaactgtacctctatagaagctatgagcaaatgtgtatggcagatgtc(+TTT)cagaggcgacgaagcgccattgactttgggaccagggagtgaaataatgctttcagatacatgtacagaaatgtctgcagtcacgcttttaaagacaaatggatactggtcagGTGTGTATGCAATCATATGAATAC NGG 10 8 17 8 24 0 100 5 10
    231127003908_12o1 Ggcttagtatacattgaggcacctgcaggaaaattatgctataattcaagacaacagctgaactgtacctctatagaagctatgagcaaatgtgtatggcagatgtc(+TAGTAC)cagaggcgacgaagcgccattgactttgggaccagggagtgaaataatgctttcagatacatgtacagaaatgtctgcagtcacgcttttaaagacaaatggatactggtcagGTGTGTATGCAATCATATGAATAC NGG 10 8 17 8 24 0 100 5 10
    231127003832_0x5o Ggcttagtatacattgaggcacctgcaggaaaattatgctataattcaagacaacagctgaactgtacctctatagaagctatgagcaaatgtgtatggcagatgtc(+TTT)cagaggcgacgaagcgccattgactttgggaccagggagtgaaataatgctttcagatacatgtacagaaatgtctgcagtcacgcttttaaagacaaatggatactggtcagGTGTGTATGCAATCATATGAATAC NGG 10 8 17 8 24 0 100 5 10
    231126234459_baxd Ggcttagtatacattgaggcacctgcaggaaaattatgctataattcaagacaacagctgaactgtacctctatagaagctatgagcaaatgtgtatggcagatgtc(-cagag)gcgacgaagcgccattgactttgggaccagggagtgaaataatgctttcagatacatgtacagaaatgtctgcagtcacgcttttaaagacaaatggatactggtcagGTGTGTATGCAATCATATGAATAC NGG 10 8 17 8 24 0 100 5 10
    231126234458_1hvo Ggcttagtatacattgaggcacctgcaggaaaattatgctataattcaagacaacagctgaactgtacctctatagaagctatgagcaaatgtgtatggcagatgtc(-cagag)gcgacgaagcgccattgactttgggaccagggagtgaaataatgctttcagatacatgtacagaaatgtctgcagtcacgcttttaaagacaaatggatactggtcagGTGTGTATGCAATCATATGAATAC NGG 10 8 17 8 24 0 100 5 10
    231126233533_vab2 cacactcaatgttaagactctaggcttagtatacattgaggcacctgcaggaaaattatgctataattcaagacaacagctgaactgtacctctatagaagctatgagcaaatgtgtatggcagatgtc(-cagag)gcgacgaagcgccattgactttgggaccagggagtgaaataatgctttcagatacatgtacagaaatgtctgcagtcacgcttttaaagacaaatggatactggtcaggaacatacag NGG 10 8 17 8 24 0 100 5 10
    231126213358_q54h GTGATCTGTTCTCAGCCTGAAAGTTACAACCTCCGCGAGGCTTTGTGTGACGGGTCGCCGGAGGGGCCTTTACTGCGCAATCC(/T)CGGGGACCACGACCGGATCCGGGTACCGCGGCTGCCCACATCCGCCGATGTAGAGTCGGTGCTGCGGCTGACGGACTACGAGACCGGGCAGATGGACCGACGGGCA NGG 10 8 17 8 24 0 100 5 10