Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231121203534_1ik5 ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTTCC(+AT)CTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203525_8bg7 ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTTCC(+AA)CTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203420_41pz ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTTCC(+A)CTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203341_7mbt ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTTCC(+A)CTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203331_7voi ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTTCC(+G)CTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203322_m27h ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTTCC(+C)CTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203312_cg80 ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTTCC(+T)CTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203256_658d ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACG(+G)ACTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203212_5ta4 ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACG(+A)ACTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203204_u9sx ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACG(+C)ACTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10