Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231118153541_li6t ttgtgtgtttatatatacatttcctcacatacaatttgtgcatttttgaaatttcagactctgagtagatcttgaacccatatcctactttgtctcaatgaaatgctcagtggtagaaactccagcattgcttatgagggagtgtttttttaagccacttcttcattgtctccctctattcaggagcagatggaagagttgaagcaaaaggcatcaaaggtgatccaggctccagaggatctccggggaaacatggcccaaagggatccattggtcctacaggagagcaa(+TAG)gggctgccaggagagactggccctcaggggcagaagggggataaaggcgaagtgggccccactggacccgaaggactaatgggcagtactggtcctttgggtcccaagggcttacctggcccgatgggccccatcggcaaaccaggtcccaggggagaagctggacccatgggccccc NGG 10 8 17 8 24 0 100 5 10
    231118113004_rvf7 ctgcctctgctcgactatataagtgccccccctctgcgcatctattgatttcagcattgcttctgatcagaagaaga(-tcccggaagagaccaaatcagaagctaaacctatctaccacaaggccaagttacgttagaccgttttgatcagtgaattagctttcaagcagttctactgctcccagccgctcgatcgatcgatcgatcaaacaaggtagctagctagctcaggatcaaatctggtttgtctacatatataggtagtgatcgatcagtcggtcggtc)atggaggctcttagcgggcgagtgggggtgaagtgtgggcggtggaacccgacggcggagcaggtgaaGGTCCTGACGGAGC NGG 10 8 17 8 24 0 100 5 10
    231118045908_bn7a TTTTATCTTTCTCTCGACCACTTAAAACTTCAGACTTCCTGTCCTGCTGGTATCATGGAGAAAGTCCAATACCTCACTCGCTCAGCTATAAGA(+aga)GCCTCAACCATTGAAATGCCTCAACAAGCACGTCAAAAGCTACAGAATCTATTTATCAATTTCTGTCTCATCTTAATATGTCTCTTGCTGATCTGTATCATCGTGATGCTTCTCTGAAGTTCTGCTACAACCTCTAGATCTGCAGCTTGCCACATCAGCTTAAAATCTGTCATCCCATGCAGAC NGG 10 8 17 8 24 0 100 5 10
    231117113920_h8d1 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231117020507_p9cq ATGATAATTGGAGGCAAGTGAATCCTGAGCGTGATTTGATAATGACCTAATAATGATGGGTTTTATTTCCAGACTTCACTTCTAATGGTGATTATGGGAGAACTGGAGCCTTCAGAGGGTAAAATTAAGCACAGTGGAAGAATTTCATTCTGTTCTCAGTTTTCCTGGATTATGCCTGGCACCATTAAAGAAAATATCAT(+CTT)TGGTGTTTCCTATGATGAATATAGATACAGAAGCGTCATCAAAGCATGCCAACTAGAAGAGGTAAGAAACTATGTGAAAACTTTTTGATTATGCATATGAACCCTTCACACTACCCAAATTATATATTTGGCTCCATATTCAATCGGTTAGTCTACATATATTTATGTTTCCTCTATGGGTAAGCTACTGTGAATGGATC NGG 10 8 17 8 24 0 100 5 10
    231116154532_b287 GCTGCTGGTTGTCTACCCCTGGACTCAGAGG(-TTCT)TCGAGTCCTTTGGGGACCTGTCCAATGCCGATGCCGTCATGGGCAATCCCAAGGTGAAGGCCCACGGCAAGAAGGTGCTCCAGTCCTTCAGT NGG 10 8 17 8 24 0 100 5 10
    231115161042_1qez GCTGCTGGTTGTCTACCCCTGGACTCAGAGG(-TTCT)TCGAGTCCTTTGGGGACCTGTCCAATGCCGATGCCGTCATGGGCAATCC NGG 10 8 17 8 24 0 100 5 10
    231115160623_sfar CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231115150440_4dg4 GTCCCCCAGACTCACCCTGAAG(-TTCT)CAGGATCCACGTGCAGCTGGTCACAGTGCAGCTCGCTCAGCTTAGCA NG 10 8 17 8 24 0 100 5 10
    231115093557_ep9p AGGGATCCACCATACACAGATGCTTCATTCCTGTCGGCCATTACTGGACTGGACTAGAACACTAAGTCCTGATAAGACACATCATTTGCAATGAGAATATATTCTGTTTGTAAGCCTTTCTGATGTGCCATTATTGCTGCTGTGTTTCCTGTTTTATGTAGGTGGTAGCCCTCGGAGAGGTACCAGATGGGACTGTGGTTACTGTCATGGCGGGTAACGATGAAAATTATTCTGCTGAGCTCCGGAATGCCTC(-T)GCTGTTATGAAAAACCAAGTAGCAAGGTTCAACGATCTGAGATTTGTGGGCCGGAGTGGACGAGGTAGGTCTCTGACTTTTGATACTGATAATAGAATAAGCACATTAGGTCCCTTTTGATG NGG 10 8 17 8 24 0 100 5 10