Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231121201412_megj ACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATG(+C)GACGACTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGA NGG 10 8 17 8 24 0 100 5 10
    231121182749_kfks ccggccgtcccagagcagtgcccacacagtgcgacgtcccccccaacagccgcttcgattgcgcccctgacaaggccatcacccaggaacagtgcgaggcccgcggctgttgctacatccctgcaaagcaggggctgcagggagcccagatggggcagccctggtgcttcttcccacccagctaccccagctacaagctggagaacctgagctcctctgaaatgggctac(/G)acggccaccctgacccgtaccacccccaccttcttccccaaggacatcctgaccctgcggctggacgtgatgatggagactgagaaccgcctccacttcacggtgggcagggcaggggcgggggcggcgg NGG 10 8 17 8 24 0 100 5 10
    231121171708_vhql gagggtggggcagatcatgcccattttgtgagtgtgcagcctgagggcccagggtcacagcttcaggtgatgcaggccctgtgggaagtggggcctccggaccccaggccagtgctccctctattcggcacaagcccttccttgacagtccccagctgtgctcaagtac(/g)atgggcgactacccgtccaagaggacacgctccgtcaacgagctcaccgaccagatctttgagggtcccctgaaagccgagcccctgaaggacgaggcatatgtgcagatcctgaagcagctgaccgacaaccacatcaggtgagccaggcacagtgggcggatgaggggcagacctcacctgctgtgacg NGG 10 8 17 8 24 0 100 5 10
    231121162626_x2wo cacaaaatcaaagaagtcttataacctaggaagtcctttcttcactttcttctcccaaacacctacaaaatttgcttcctcctttgtgtctttacccaaatttaatctctatatgtctttctctaaccaacttatttctactcatccctgccaccagtgcgtctgtaatacttattaacttgtaatatattacataaggtaagctccatgagggtagA(/T)aattttgttctttctattttctgtttttcctccagcccctggaacaatacctgacacagagtaagcactcaataaataattggtgaaggcattaatcccagaggcagttcagcaagaggctgatggagtggttctgggactc NGG 10 8 17 8 24 0 100 5 10
    231121134529_his1 ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGATG(/A)GGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCG NGG 10 8 17 8 24 0 100 5 10
    231120135016_iehu CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231118155717_5yp2 attactttacctaccatatcactgtgttctccaggaatgtgcaggtatctttggtcaaaaacggggtaaaagtcctgcacaccaaggacagttacatgagctctgaggaccaggcgtctggtggcattgtgcaggagctgaaactcggggacgaagtgtggatgcaggtg(+TAG)acaggaggagagaggttcaatggcttatttgcagacgaggatgacgataccacgttcacgggcttcctgctgttcagcagctcttgacacaaggactctgcacgtgggcctcacagagggctctctctgctggtgaacgtggtgcctacagaactcgcttggggagggtgggatgtgactg NGG 10 8 17 8 24 0 100 5 10
    231118155324_8h7v tgatcagtaagttccctcccccagatgcacccattaaattcgataagatcctatacaatgaactgaaccactacaatgtagcgacggggaagttcacctgccacgtggcaggtgtctattactttacctaccatatcactgtgttctccaggaatgtgcaggtatctttggtcaaaaacggggtaaaagtcctgcacaccaaggacagttacatgagctct(+TAG)gaggaccaggcgtctggtggcattgtgcaggagctgaaactcggggacgaagtgtggatgcaggtgacaggaggagagaggttcaatggcttatttgcagacgaggatgacgataccacgttcacgggct NGG 10 8 17 8 24 0 100 5 10
    231118154956_pxzf agggggagccaggagtcagaggaatgagaggctggaaaggcgatcgaggagagaaggggaaagttggtgaggctccccttgtgcccaagagtgctttcactgtgggactcacggtgatcagtaagttccctcccccagatgcacccattaaattcgataagatcctatacaatgaactgaaccactacaatgtagcg(+TAG)acggggaagttcacctgccacgtggcaggtgtctattactttacctaccatatcactgtgttctccaggaatgtgcaggtatctttggtcaaaaacggggtaaaagtcctgcacaccaaggacagttacatgagctctgaggaccaggcgt NGG 10 8 17 8 24 0 100 5 10
    231118154419_xlib gacccgaaggactaatgggcagtactggtcctttgggtcccaagggcttacctggcccgatgggccccatcggcaaaccaggtcccaggggagaagctggacccatgggcccccagggggagccaggagtcagaggaatgagaggctggaaaggcgatcgaggagagaaggggaaagttggtgaggctccccttgtgcccaagagtgctttcact(+TAG)gtgggactcacggtgatcagtaagttccctcccccagatgcacccattaaattcgataagatcctatacaatgaactgaaccactacaatgtagcgacggggaagttcacctgccacgtggcaggtgtctatt NGG 10 8 17 8 24 0 100 5 10