Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231121203154_r0yk ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACG(+T)ACTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121203141_iy87 ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACG(+G)ACTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121202323_kmjc ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTTC(+G)CCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121202154_gmb4 ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATG(-GACGA)CTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121202126_9c5p ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTT(-CC)CTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121202110_14xh ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACAC(-TC)TTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121202047_geqi ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGACGACTACACTCTTC(+T)CCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121202026_lvrv ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATGGAC(-G)ACTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121201952_mabp ATAAGACAGAAAACAGACAGACGGGTAAATCTGAAAGTAAAGAAACAGACAGGTGAATCAGGAGTCTCCTCTCACTCTCCTCTAATGATG(+C)GACGACTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    231121201614_zxiu ATGATGGACGACTACACTCTTCCCTTCTGGATTTACCTGGGTGTTCTGACCGTGTTCATTGTTGGAGCTATGAAGAAGATATTGGCATCCCACCTGAGCGCCGCCCC(+T)CCCGCTGGTGGCCTGGCTGGGGGCCACCGTGCTGGTGGAGCGGCTGTGGGCGTTCTGCCTGCCGGCCGTGCTGCTGCTGGTGCTGCTCGGCCTCACCTGCTGCTTCTACTCTGCTGCCAGGGCGCCGCCGCCCGCTGCCCTGCCGGCCCACGGCAAGGCCGTCTTGATCACAG NGG 10 8 17 8 24 0 100 5 10