Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153756_remf CAGGGAGGCTTGGTAGTTTCTCTGTGTAATAG(+AGGACA)GATCCAGTGAACTTCAGGTTTCTCCTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613153755_h7kf GGAGAAACAAAGGAGAAACCTGAAGTTCACTG(+AGGACA)GATCCTATTACACAGAGAAACTACCAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240613153752_5j8v GAGACTCCTGCAAAGGCTATGAAACTCCCAGG(+AGGACA)GATCCCAGAGGGCTGGAATCCTGAGATGTCC NGG 10 8 17 8 24 0 100 5 10
    240613153751_iopk ATGCCGGACATCTCAGGATTCCAGCCCTCTGG(+AGGACA)GATCCCTGGGAGTTTCATAGCCTTTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613153749_mis3 GGTCTTATATGATAATAACTACAGTAAGTGTG(+AGGACA)GTACCCAGACTGAAAGATCAATACATGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613153748_dyz1 GACATAGAGCATGTATTGATCTTTCAGTCTGG(+AGGACA)GTACCACACTTACTGTAGTTATTATCATATA NGG 10 8 17 8 24 0 100 5 10
    240613153745_vtf3 TATTAGAGCAAGAGGCAGAGACTATGACCCAG(+AGGACA)GTACCCCAGGGAAGATTCAGTCTAGAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240613153742_8sr5 CAAAATTACCTCTAGACTGAATCTTCCCTGGG(+AGGACA)GTACCTGGGTCATAGTCTCTGCCTCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613153740_ttpx GGCCCCAGAGGGAGGTGCAATCGACCTTTAGG(+AGGACA)GATCCTCACCGACCAACTCAGCGAGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240613153739_9ol7 TCCTCTTCTGTCTCGCTGAGTTGGTCGGTGAG(+AGGACA)GATCCCTAAAGGTCGATTGCACCTCCCTCTG NGG 10 8 17 8 24 0 100 5 10