Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153810_v49v CCCCTAATTATTATTATTTTTTACATGTGATG(+AGGACA)GTACCATAACATTTCTGGGACTTCTACAGCA NGG 10 8 17 8 24 0 100 5 10
    240613153809_k2kr TACATTGCTGTAGAAGTCCCAGAAATGTTATG(+AGGACA)GTACCATCACATGTAAAAAATAATAATAATT NGG 10 8 17 8 24 0 100 5 10
    240613153808_ndr0 TAGTTTATAAACAGGCATCCTGGAGTTAGCAG(+AGGACA)GTACCTCTCCCATAGCATTATATTTTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240613153806_lawu ATAATTTTGGAAAATATAATGCTATGGGAGAG(+AGGACA)GTACCTGCTAACTCCAGGATGCCTGTTTATA NGG 10 8 17 8 24 0 100 5 10
    240613153805_4yzw CAGCCTGATACAACAGGCAGTGTCCTTCTTCG(+AGGACA)GATCCCATCTGGGGATGCTGCTCGCCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240613153803_slqr GAGGTGTCTGGGCGAGCAGCATCCCCAGATGG(+AGGACA)GATCCGAAGAAGGACACTGCCTGTTGTATCA NGG 10 8 17 8 24 0 100 5 10
    240613153801_0jzu TATCACATAGTCGCAGGTCATAAAGGAATGGG(+AGGACA)GATCCAAAAGCAGTTTTCATCTTCAGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240613153800_gsx7 CTCTAAGACTCTGAAGATGAAAACTGCTTTTG(+AGGACA)GATCCCCATTCCTTTATGACCTGCGACTATG NGG 10 8 17 8 24 0 100 5 10
    240613153759_zcxm TCTCTCTTGTAACTTAGAATACAATGAAAATG(+AGGACA)GATCCAGCCATCATGGGGTTATTGGGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613153757_qihp AAACAACTTTCCCAATAACCCCATGATGGCTG(+AGGACA)GATCCATTTTCATTGTATTCTAAGTTACAAG NGG 10 8 17 8 24 0 100 5 10