Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153907_teb6 CAGGACTGCTTGAACCCAAGTTTGGTCTTTGG(+AGGACA)GATCCACATAGTGGAAAGACACACAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240613153906_tdrs CCTGCCAGAATTGTGTGTCTTTCCACTATGTG(+AGGACA)GATCCCAAAGACCAAACTTGGGTTCAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613153904_oxf3 TACCAACATCTTAAACGGACTTTCCTGTGGTG(+AGGACA)GTACCCTGAGAGGCGGATGAAAGCTACTCTG NGG 10 8 17 8 24 0 100 5 10
    240613153902_xn4l ACTGACAGAGTAGCTTTCATCCGCCTCTCAGG(+AGGACA)GTACCACCACAGGAAAGTCCGTTTAAGATGT NGG 10 8 17 8 24 0 100 5 10
    240613153901_leal GCTGTCTTCAGACACACCAGAAGGCAGCCTCG(+AGGACA)GATCCCATTACAGATGGCTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613153859_5myz ACCACATGGTGGCTCACAGCCATCTGTAATGG(+AGGACA)GATCCGAGGCTGCCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613153857_ctjv TTAAAAGTATTATATTTCTAAAATTATGTGAG(+AGGACA)GATCCTTTTTTAATTCAGCTCATTAATATGA NGG 10 8 17 8 24 0 100 5 10
    240613153856_gw80 CGTTTTCATATTAATGAGCTGAATTAAAAAAG(+AGGACA)GATCCTCACATAATTTTAGAAATATAATACT NGG 10 8 17 8 24 0 100 5 10
    240613153855_vzrc GCCGGCCGTGGCTTTCTCAGAGAGGACTCCAG(+AGGACA)GTACCCACACTGTCAGGCTGCCTCCCGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613153853_wmlo CATGTGCCCCGGGAGGCAGCCTGACAGTGTGG(+AGGACA)GTACCTGGAGTCCTCTCTGAGAAAGCCACGG NGG 10 8 17 8 24 0 100 5 10