Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153950_xyv5 GCAGTTCCCACCTGTGGCCATGCTCAGCACTG(+AGGACA)GTACCCAGCCGATTTTCAGTCAGGCTTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613153948_oesv AAAGGCAGAAAGCCTGACTGAAAATCGGCTGG(+AGGACA)GTACCAGTGCTGAGCATGGCCACAGGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613153947_cn6y CCTTGGGTTTCATTTAGCTTCTCACAGTCAAG(+AGGACA)GATCCAAGACGCCAAATCTATGTGTGAGCAT NGG 10 8 17 8 24 0 100 5 10
    240613153946_9ze8 GTCAAATGCTCACACATAGATTTGGCGTCTTG(+AGGACA)GATCCTTGACTGTGAGAAGCTAAATGAAACC NGG 10 8 17 8 24 0 100 5 10
    240613153945_7435 GTATTTAGAATGTTATATTTATTGTCGGTTTG(+AGGACA)GTACCACCTTGTCCCAGGTGAGCATTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240613153943_n9iy CCATTGGCCAAATGCTCACCTGGGACAAGGTG(+AGGACA)GTACCAAACCGACAATAAATATAACATTCTA NGG 10 8 17 8 24 0 100 5 10
    240613153942_s5vo AAAGAGGGGGAGGAATGAGACAGGACAGGCAG(+AGGACA)GATCCCTGAAATAACTGGAACGCACAATAAG NGG 10 8 17 8 24 0 100 5 10
    240613153940_4eq5 GCCATCTTATTGTGCGTTCCAGTTATTTCAGG(+AGGACA)GATCCTGCCTGTCCTGTCTCATTCCTCCCCC NGG 10 8 17 8 24 0 100 5 10
    240613153938_sase GAGTGTCAGGACTCTGAAGGAAGGGCAGCCAG(+AGGACA)GTACCAAGCTACCTCCTTCCCACCCTCAACC NGG 10 8 17 8 24 0 100 5 10
    240613153937_ku2h AGTTGGGTTGAGGGTGGGAAGGAGGTAGCTTG(+AGGACA)GTACCTGGCTGCCCTTCCTTCAGAGTCCTGA NGG 10 8 17 8 24 0 100 5 10