Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153921_pne8 AGAGCCAAAGAGCCAGGAGCTAAGCCCAAAGG(+AGGACA)GATCCACAAACTGCAGCCGACCCGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613153920_r1x2 TTAAATCCACTGCGGGTCGGCTGCAGTTTGTG(+AGGACA)GATCCCTTTGGGCTTAGCTCCTGGCTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613153918_u3gy TCCAACCACGGCACCGGAAAAGGCTGTGCAAG(+AGGACA)GATCCAACGCAGAGGCTGCTAGAGCGGATGC NGG 10 8 17 8 24 0 100 5 10
    240613153917_8kk4 TCGAAGCATCCGCTCTAGCAGCCTCTGCGTTG(+AGGACA)GATCCTTGCACAGCCTTTTCCGGTGCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240613153916_f55u AGCCCTCTTCTGGCCTTCTCAGGGCCTTTTTG(+AGGACA)GATCCCTGAAACAACACATTCCCTGACCTTT NGG 10 8 17 8 24 0 100 5 10
    240613153914_s91c GACAGAAAGGTCAGGGAATGTGTTGTTTCAGG(+AGGACA)GATCCAAAAAGGCCCTGAGAAGGCCAGAAGA NGG 10 8 17 8 24 0 100 5 10
    240613153913_bqge TGCATACCTTCCTTCAAGTTTTGTCCTTGTAG(+AGGACA)GTACCCTCCTTTTGATTAAGAGGTGGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613153911_4oma TACACAACCTCCACCTCTTAATCAAAAGGAGG(+AGGACA)GTACCTACAAGGACAAAACTTGAAGGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240613153910_d10o CTACCCATATCCAGCAGCTCTGCCTTTCTCAG(+AGGACA)GTACCTCCTGAAGGTCCTGCCATCCTGCCGA NGG 10 8 17 8 24 0 100 5 10
    240613153909_vltb AAGACTCGGCAGGATGGCAGGACCTTCAGGAG(+AGGACA)GTACCTGAGAAAGGCAGAGCTGCTGGATATG NGG 10 8 17 8 24 0 100 5 10