Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154004_ip48 AAAATTTGAAAATATTGACTCCTGAGTGGGAG(+AGGACA)GTACCACACCTGAACCAACCCACAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613154003_c5y7 CCGGCCAGCGTTTGTGGGTTGGTTCAGGTGTG(+AGGACA)GTACCTCCCACTCAGGAGTCAATATTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240613154001_oibb AATGTATCAAATCATGAAGCAAGGTGGCCAAG(+AGGACA)GATCCCATAGCCTGGTCACAGTGGGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613153959_429w GAGGTCTGCTTCCCACTGTGACCAGGCTATGG(+AGGACA)GATCCTTGGCCACCTTGCTTCATGATTTGAT NGG 10 8 17 8 24 0 100 5 10
    240613153958_bvd6 ATTTTAGATGTTTAAGATAAGAAACTAGCTTG(+AGGACA)GTACCATTAAAATGCCCACTATGTCCTACTG NGG 10 8 17 8 24 0 100 5 10
    240613153957_5aaq GTGATCAGTAGGACATAGTGGGCATTTTAATG(+AGGACA)GTACCAAGCTAGTTTCTTATCTTAAACATCT NGG 10 8 17 8 24 0 100 5 10
    240613153955_76mt CAGTTTTCCGCATGATGTCTTCTGTGATGCAG(+AGGACA)GTACCCTTATTCATTTGTGATTTTCAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240613153954_lqyy GTGCTATTCTTGAAAATCACAAATGAATAAGG(+AGGACA)GTACCTGCATCACAGAAGACATCATGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240613153952_j2xp TTTGCTATAGTCACTAGGGCCATTTACTATAG(+AGGACA)GATCCATTGTAATGAGAATGTTCATAAACTG NGG 10 8 17 8 24 0 100 5 10
    240613153951_hu5n CATGTCAGTTTATGAACATTCTCATTACAATG(+AGGACA)GATCCTATAGTAAATGGCCCTAGTGACTATA NGG 10 8 17 8 24 0 100 5 10