Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154109_fngb TATCAGCCAGTGTGGCCCTCTATTTCCTTGTG(+AGGACA)GTACCGAGTGTCCCTGAGGCTGGGGTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240613154108_ngqv GAAAAAGACCACCCCAGCCTCAGGGACACTCG(+AGGACA)GTACCACAAGGAAATAGAGGGCCACACTGGC NGG 10 8 17 8 24 0 100 5 10
    240613154106_pyox AGCATGCAGACATCCCTCTGTGGACTTCAGGG(+AGGACA)GATCCACCTCTAAAGAGAGTCACTAGTGACC NGG 10 8 17 8 24 0 100 5 10
    240613154104_edm7 CTGTTGGTCACTAGTGACTCTCTTTAGAGGTG(+AGGACA)GATCCCCTGAAGTCCACAGAGGGATGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613154103_nunc GGGTTCCCACGTGTCTGGAAGCTCGAGTTCCG(+AGGACA)GATCCATACTGACACCCAATAAGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240613154101_z3dv TACTGCTACACAGCTTATTGGGTGTCAGTATG(+AGGACA)GATCCGGAACTCGAGCTTCCAGACACGTGGG NGG 10 8 17 8 24 0 100 5 10
    240613154100_j1wt AGACTCGGCCAGAGACAGACGTTAGAGGACGG(+AGGACA)GATCCAGTCCCTCTCCCACAGCAGGCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240613154058_0kkn TAAGTGCTTTGCCTGCTGTGGGAGAGGGACTG(+AGGACA)GATCCCGTCCTCTAACGTCTGTCTCTGGCCG NGG 10 8 17 8 24 0 100 5 10
    240613154057_7c1h GCAGAACCTAGGGCTTTGACCATGGCTTAGTG(+AGGACA)GTACCATGCGAAGACACTTAGTGTGACAAGT NGG 10 8 17 8 24 0 100 5 10
    240613154056_nvzc CTCAGACTTGTCACACTAAGTGTCTTCGCATG(+AGGACA)GTACCACTAAGCCATGGTCAAAGCCCTAGGT NGG 10 8 17 8 24 0 100 5 10