Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154210_tsgb ACCCTTTCTCTGACACACAGAGAGAGCTGCAG(+AGGACA)GTACCTAGCTGAGAACCAGGGCATGGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613154208_nk2k ATCCCCCTTCCCATGCCCTGGTTCTCAGCTAG(+AGGACA)GTACCTGCAGCTCTCTCTGTGTGTCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613154207_ybjn ATTATTATTATTATTACTTGTGTGTGTGTGTG(+AGGACA)GATCCCACATCCTCCAACAGCTCCAGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240613154205_l72w CTTCCAACCCCTGGAGCTGTTGGAGGATGTGG(+AGGACA)GATCCACACACACACACAAGTAATAATAATA NGG 10 8 17 8 24 0 100 5 10
    240613154204_sbck CTCAAAGCTTTGAGGAGTGAAGTTAGGAGTAG(+AGGACA)GTACCAGTGACTACGAACTGAACATGCACTG NGG 10 8 17 8 24 0 100 5 10
    240613154203_u8v3 GCTCACAGTGCATGTTCAGTTCGTAGTCACTG(+AGGACA)GTACCTACTCCTAACTTCACTCCTCAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240613154201_sdci GTAGTGATAAACTTCTGGTGGGATAGAAAGAG(+AGGACA)GATCCATCTTCAAACCTCAAGTCTTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613154200_0kli TCCCATCCAGGAAGACTTGAGGTTTGAAGATG(+AGGACA)GATCCTCTTTCTATCCCACCAGAAGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240613154159_6gs9 ATTAAGAAGAGATTTTTTTAATCTATTGAATG(+AGGACA)GATCCTCATTTGTAAAAATTTTTAGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240613154158_ahy7 AACAAAAATGGCTAAAAATTTTTACAAATGAG(+AGGACA)GATCCATTCAATAGATTAAAAAAATCTCTTC NGG 10 8 17 8 24 0 100 5 10