Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154225_y7qt TCTGTCATAATGTAGATCATGGTAGGTATGTG(+AGGACA)GATCCCCTAAACATCTTCACATAGTGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613154223_vuvz CATTGGAGCACACTATGTGAAGATGTTTAGGG(+AGGACA)GATCCACATACCTACCATGATCTACATTATG NGG 10 8 17 8 24 0 100 5 10
    240613154222_ct4f TCTATTCCTGCCGAAGCTTGCCAGGGCAGGTG(+AGGACA)GTACCCAAGAGGCTCTTCCTTTCCTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613154220_3nli CTTCTCTTCAGAGGAAAGGAAGAGCCTCTTGG(+AGGACA)GTACCACCTGCCCTGGCAAGCTTCGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613154218_ddun CTGTATTAAGCAGAACCCAGGTCGTTTGCTTG(+AGGACA)GATCCACGATTTGACTTCAAGTTGTTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613154217_vm9n TTCGAAGACAAACAACTTGAAGTCAAATCGTG(+AGGACA)GATCCAAGCAAACGACCTGGGTTCTGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613154216_5okt TTACAGACTGGGTGGTAAAGAGGGGCATCTTG(+AGGACA)GTACCTGGGTCCCAGAGAGAGAAGTCAAACA NGG 10 8 17 8 24 0 100 5 10
    240613154213_b1k4 CATTTTGTTTGACTTCTCTCTCTGGGACCCAG(+AGGACA)GTACCAAGATGCCCCTCTTTACCACCCAGTC NGG 10 8 17 8 24 0 100 5 10
    240613154212_18rd TAGGGCTCACTTGTATATTAGTTTCTGAATTG(+AGGACA)GATCCATAACTTTAAGATTAAACAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613154211_kqga TTATCTTTTCTCTGTTTAATCTTAAAGTTATG(+AGGACA)GATCCAATTCAGAAACTAATATACAAGTGAG NGG 10 8 17 8 24 0 100 5 10