Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154122_byhy TCACACAGCACTTACAATGTGGCCACACCAAG(+AGGACA)GTACCATGCCAGTCTATATGAAAACAACAGA NGG 10 8 17 8 24 0 100 5 10
    240613154121_gbkn AGCAGTCTGTTGTTTTCATATAGACTGGCATG(+AGGACA)GTACCTTGGTGTGGCCACATTGTAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613154119_znw2 GTGGGCACAGCTAAGGTCCATTTTAACTCTGG(+AGGACA)GATCCATCTCAGCATACCTTTCAACAATTAT NGG 10 8 17 8 24 0 100 5 10
    240613154118_zbgt GTTACATAATTGTTGAAAGGTATGCTGAGATG(+AGGACA)GATCCCAGAGTTAAAATGGACCTTAGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613154117_j5tp TCAATATTTAAATCATTCAGGATTATTATAAG(+AGGACA)GATCCAGTGAAATAGAGCAGACAGAAGTAAA NGG 10 8 17 8 24 0 100 5 10
    240613154115_o32a CATTTTTTACTTCTGTCTGCTCTATTTCACTG(+AGGACA)GATCCTTATAATAATCCTGAATGATTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240613154114_mnfw CTCCATCCTTGCACTCGCCACTACCCTAGAAG(+AGGACA)GATCCTTCTGCTGCCCTCTGCCTCCCATCCG NGG 10 8 17 8 24 0 100 5 10
    240613154113_nx5w TGGGGCGGATGGGAGGCAGAGGGCAGCAGAAG(+AGGACA)GATCCTTCTAGGGTAGTGGCGAGTGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613154112_3k4j TATTTTGGAATAAAATTTATTTTATGGCTTAG(+AGGACA)GATCCATGGCCCCCTTCCCCTCCAGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240613154110_yd38 TTTTTCCCTCACTGGAGGGGAAGGGGGCCATG(+AGGACA)GATCCTAAGCCATAAAATAAATTTTATTCCA NGG 10 8 17 8 24 0 100 5 10