Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154154_o4l0 CCCCTGTCCTGCTGATGCTGTCTATCCCCTGG(+AGGACA)GATCCAGGGTCCTCTGCCTGCCCTCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240613154152_xo13 CTTTTCCAAAGGAGGGCAGGCAGAGGACCCTG(+AGGACA)GATCCCAGGGGATAGACAGCATCAGCAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613154151_lpz0 TTCCTGACGGCTGATGTGGGCCAGTGGCAGAG(+AGGACA)GATCCAGGTTGCCCTTCCTTCCCCCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240613154149_13la TTCCTGTGGTGGGGGGAAGGAAGGGCAACCTG(+AGGACA)GATCCTCTGCCACTGGCCCACATCAGCCGTC NGG 10 8 17 8 24 0 100 5 10
    240613154146_dztj TGGGACACTTGGCAGAAGGGATTGCCCTGGGG(+AGGACA)GTACCAAGAGCCTAGGGTGCTCCCTGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240613154145_z2bf CTGGGACCTACAGGGAGCACCCTAGGCTCTTG(+AGGACA)GTACCCCCAGGGCAATCCCTTCTGCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240613154143_6a1t CCTGCGTCTCCCAGCATGGTAGGTAGCCTCAG(+AGGACA)GATCCTAGAAAGATTATGGCCAGTGTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240613154142_0men GTCAGAACAGACACTGGCCATAATCTTTCTAG(+AGGACA)GATCCTGAGGCTACCTACCATGCTGGGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613154140_hqd8 CAAGCAACTGGTAGCTCTCACAATTCTGTTGG(+AGGACA)GATCCTTTGTGCAATATTGTAGCCATCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613154139_gruf CCAGGGGAAGATGGCTACAATATTGCACAAAG(+AGGACA)GATCCCAACAGAATTGTGAGAGCTACCAGTT NGG 10 8 17 8 24 0 100 5 10