Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155627_815i CTAGTTAACTCACTCCCATAATGTTCCTTTGG(+AGGACA)GTACCAGCTGTAAGCCAACACCACTCACAAA NGG 10 8 17 8 24 0 100 5 10
    240613155625_z0v4 TAATTTTTGTGAGTGGTGTTGGCTTACAGCTG(+AGGACA)GTACCCAAAGGAACATTATGGGAGTGAGTTA NGG 10 8 17 8 24 0 100 5 10
    240613155623_5ii1 GAAAGGGTAGATGGTGCCAGTTATTTGCCCTG(+AGGACA)GTACCTTTGGGCCCAGGAAGTTCATCACGGT NGG 10 8 17 8 24 0 100 5 10
    240613155621_c1t9 TGCACACCGTGATGAACTTCCTGGGCCCAAAG(+AGGACA)GTACCAGGGCAAATAACTGGCACCATCTACC NGG 10 8 17 8 24 0 100 5 10
    240613155620_qacz TCTCTTCCCCTAGGATGTTTCTCAATTGAGTG(+AGGACA)GATCCCAGCAGTTTACATTTGCCACAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240613155618_4pfv GTCTGTAACTTGTGGCAAATGTAAACTGCTGG(+AGGACA)GATCCACTCAATTGAGAAACATCCTAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613155617_4fqj AAGGCAGTGACTCTCAACCTTCCTAATGCAGG(+AGGACA)GATCCTTCAATACAATTCCTTATTTGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613155615_t8t3 AGGATCACCACAAATAAGGAATTGTATTGAAG(+AGGACA)GATCCCTGCATTAGGAAGGTTGAGAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240613155614_b2qi AGCTAGGGGCAGGCTTATGATACACTTGGAAG(+AGGACA)GATCCAAGGGACAGGGCAGTGTGGCCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613155611_6cac TCTCTCCAGGGGCCACACTGCCCTGTCCCTTG(+AGGACA)GATCCTTCCAAGTGTATCATAAGCCTGCCCC NGG 10 8 17 8 24 0 100 5 10