Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154138_psqz TCTAGGGGCAAGCCAGCTCAAGCAGCCTGAAG(+AGGACA)GATCCCGGATTGGTGGCTGTTGGAATGAAGA NGG 10 8 17 8 24 0 100 5 10
    240613154136_crkb TCATTTCTTCATTCCAACAGCCACCAATCCGG(+AGGACA)GATCCTTCAGGCTGCTTGAGCTGGCTTGCCC NGG 10 8 17 8 24 0 100 5 10
    240613154135_1j6d GCATGTATCAATACACATTACATTCATGCTTG(+AGGACA)GTACCTGTGGAGGTCAGAAGGGGGCATCCGA NGG 10 8 17 8 24 0 100 5 10
    240613154133_asr1 GGGGATCGGATGCCCCCTTCTGACCTCCACAG(+AGGACA)GTACCAAGCATGAATGTAATGTGTATTGATA NGG 10 8 17 8 24 0 100 5 10
    240613154132_49v7 TCAATGGTGATTAGAGAAGCAAGCATTATATG(+AGGACA)GATCCCAATCAGATAGAGAAATGCAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613154130_xm4n TTTCTTTTCTTTGCATTTCTCTATCTGATTGG(+AGGACA)GATCCATATAATGCTTGCTTCTCTAATCACC NGG 10 8 17 8 24 0 100 5 10
    240613154129_4trl CACTCGCTGGTCCCAACCTCCTCATATGTGTG(+AGGACA)GATCCTCAAGGAAGGCCATGATGATCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240613154127_4xt9 CCCAAGCCCTGATCATCATGGCCTTCCTTGAG(+AGGACA)GATCCACACATATGAGGAGGTTGGGACCAGC NGG 10 8 17 8 24 0 100 5 10
    240613154126_lbhx TGCAATTACTGCAATCCCTCTGAAAGTGCTTG(+AGGACA)GATCCGCCGGCAAATATTAAAAGGAGATTTC NGG 10 8 17 8 24 0 100 5 10
    240613154124_yehm TTGTGGAAATCTCCTTTTAATATTTGCCGGCG(+AGGACA)GATCCAAGCACTTTCAGAGGGATTGCAGTAA NGG 10 8 17 8 24 0 100 5 10