Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154020_g8gr CTGCTGGCATCACCACACTCAGCCAGCCCCTG(+AGGACA)GATCCTTCTGACTTGGCTTTGCCATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613154019_zgde AAAACAAAAAAATGGCAAAGCCAAGTCAGAAG(+AGGACA)GATCCAGGGGCTGGCTGAGTGTGGTGATGCC NGG 10 8 17 8 24 0 100 5 10
    240613154018_e3eq AAGGAGGCAAGTCCTAACATGGGGGTGAGCTG(+AGGACA)GATCCAGACACGGGTGGGTGCGTAGAGTGCC NGG 10 8 17 8 24 0 100 5 10
    240613154016_sxke CTCTCGGCACTCTACGCACCCACCCGTGTCTG(+AGGACA)GATCCAGCTCACCCCCATGTTAGGACTTGCC NGG 10 8 17 8 24 0 100 5 10
    240613154015_3fa4 GGGGCAGGCCAAAAGATGGATGATAAGAAGTG(+AGGACA)GATCCACTTGAGAGTGGCTGAAGAACCAAGG NGG 10 8 17 8 24 0 100 5 10
    240613154014_gczz CGCTACCTTGGTTCTTCAGCCACTCTCAAGTG(+AGGACA)GATCCACTTCTTATCATCCATCTTTTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613154012_lkwl CTTTAAGGATGTGTTAAAATCTGAAGGCATTG(+AGGACA)GATCCTGAGGAATGATCAGGAGAGGCACTGA NGG 10 8 17 8 24 0 100 5 10
    240613154010_1qnj CCTGCTCAGTGCCTCTCCTGATCATTCCTCAG(+AGGACA)GATCCAATGCCTTCAGATTTTAACACATCCT NGG 10 8 17 8 24 0 100 5 10
    240613154009_tko3 AGCCGCGAACACCAAGAGGAAAGAAAAGGATG(+AGGACA)GATCCCCCCCCCCAGTCCCCGTCTCCCATCC NGG 10 8 17 8 24 0 100 5 10
    240613154006_qkrx GTGGGGGATGGGAGACGGGGACTGGGGGGGGG(+AGGACA)GATCCATCCTTTTCTTTCCTCTTGGTGTTCG NGG 10 8 17 8 24 0 100 5 10