Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153936_okyb CAGGCCTGTTTTCCCCCACCACTCTGACTTTG(+AGGACA)GTACCCTTTGAGCCATGCACACAATAGCTTA NGG 10 8 17 8 24 0 100 5 10
    240613153934_08qz TACCTTAAGCTATTGTGTGCATGGCTCAAAGG(+AGGACA)GTACCAAAGTCAGAGTGGTGGGGGAAAACAG NGG 10 8 17 8 24 0 100 5 10
    240613153932_1mp6 TGGTGGACCCTTCCATAAAACTCATCTCCCTG(+AGGACA)GATCCAGGTTAAAGACTCAGTGGAAGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240613153930_79pb CTTCTACACACTTCCACTGAGTCTTTAACCTG(+AGGACA)GATCCAGGGAGATGAGTTTTATGGAAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613153929_4e84 AGCTTAAGGTAAGGCATCCCCCCTGCACAGAG(+AGGACA)GATCCTAGAAACTGTCCGTCAAGCTCCCTAA NGG 10 8 17 8 24 0 100 5 10
    240613153928_ga3o AGCTTTTAGGGAGCTTGACGGACAGTTTCTAG(+AGGACA)GATCCTCTGTGCAGGGGGGATGCCTTACCTT NGG 10 8 17 8 24 0 100 5 10
    240613153926_wl5y CAGGTGTTGACTGCCACCAAGGTTTGATACGG(+AGGACA)GATCCAAATTCAGGCCCCCATCTCGTCCATC NGG 10 8 17 8 24 0 100 5 10
    240613153925_lril TGCTCGATGGACGAGATGGGGGCCTGAATTTG(+AGGACA)GATCCCGTATCAAACCTTGGTGGCAGTCAAC NGG 10 8 17 8 24 0 100 5 10
    240613153924_8dxg AGCAGTTACGAGCTTGTATAACGAGGACTCTG(+AGGACA)GATCCAGATTTAAGTAGATTCAAACACCACT NGG 10 8 17 8 24 0 100 5 10
    240613153923_rwsl TGTTAAGTGGTGTTTGAATCTACTTAAATCTG(+AGGACA)GATCCAGAGTCCTCGTTATACAAGCTCGTAA NGG 10 8 17 8 24 0 100 5 10