Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153637_65da GAGCTGTTAGCTACCTCTAAGGCTCCCATTGG(+AGGACA)GATCCTGTGCTTTTTATTTACAGTAAAGCAT NGG 10 8 17 8 24 0 100 5 10
    240613153636_dc8m GACAAATGCTTTACTGTAAATAAAAAGCACAG(+AGGACA)GATCCCAATGGGAGCCTTAGAGGTAGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240613153634_jzij CTGGATGCTTCACTCCTGTACAAGTCCCGTTG(+AGGACA)GTACCATATTCGAATGTTATTCTCTTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613153632_sbum GTAAACAGAGAAGAGAATAACATTCGAATATG(+AGGACA)GTACCAACGGGACTTGTACAGGAGTGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240613153631_yiw9 ACTTATTAACAAGCCGAGCAGAATTTTTATAG(+AGGACA)GATCCATCCATCCAGATTTATTACAGAACAC NGG 10 8 17 8 24 0 100 5 10
    240613153630_m1fd AAATGGTGTTCTGTAATAAATCTGGATGGATG(+AGGACA)GATCCTATAAAAATTCTGCTCGGCTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240613153628_azt6 TCTTGAGTTGATGATAACTGGAAGTCCACCTG(+AGGACA)GTACCTGCCTTCCTGTTCATTTGTCTTCATT NGG 10 8 17 8 24 0 100 5 10
    240613153626_15hv CTTTCAATGAAGACAAATGAACAGGAAGGCAG(+AGGACA)GTACCAGGTGGACTTCCAGTTATCATCAACT NGG 10 8 17 8 24 0 100 5 10
    240613153625_2syi ATAGTAAAGTCTAAAAATGAGCAAGCCATTGG(+AGGACA)GTACCAACAGGTTCAAATGTTAGCTTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240613153623_gwnx ACACCACTGCAAGCTAACATTTGAACCTGTTG(+AGGACA)GTACCCAATGGCTTGCTCATTTTTAGACTTT NGG 10 8 17 8 24 0 100 5 10